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Literature summary extracted from

  • Mulichak, A.M.; Theisen, M.J.; Essigmann, B.; Benning, C.; Garavito, R.M.
    Crystal structure of SQD1, an enzyme involved in the biosynthesis of the plant sufolipid headgroup donor UDP-sulfoquinovose (1999), Proc. Natl. Acad. Sci. USA, 96, 13097-13102.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.1.1 expressed in Escherichia coli Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.13.1.1 sitting drop vapor diffusion results in bipyramidal crystals, C-2-symmetric homodimer, the protein has a bidomain structure, catalytic site is located in the cleft formed between the two lobes Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.13.1.1 additional information the function of different amino acids in reaction/binding process is analysed, Thr-145, Tyr-182 and Lys-186 are found to fulfill analogous mechanistic roles Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.13.1.1 chloroplast thylakoid Arabidopsis thaliana 9507
-
3.13.1.1 membrane photosynthetic Arabidopsis thaliana 16020
-
3.13.1.1 thylakoid
-
 Arabidopsis thaliana 9579
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.13.1.1 UDP-glucose + sulfite Arabidopsis thaliana sulfolipid biosynthesis UDP-sulfoquinovose + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.13.1.1 Arabidopsis thaliana O48917
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.13.1.1 Ni2+-nitrilotriacetic acid column Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
3.13.1.1 UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite contains characteristic conserved Y-XXX-K and glycine-rich sequence patterns Arabidopsis thaliana
a
3.13.1.1 UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite short-chain dehydrogenase/reductase family Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.1.1 UDP-glucose + sulfite
-
 Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?
3.13.1.1 UDP-glucose + sulfite sulfolipid biosynthesis Arabidopsis thaliana UDP-sulfoquinovose + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
3.13.1.1 NAD+
-
 Arabidopsis thaliana