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Literature summary extracted from

  • Smith, K.A.; Salyers, A.A.
    Characterization of a neopullulanase and an alpha-glucosidase from Bacteroides thetaiotaomicron 95-1 (1991), J. Bacteriol., 173, 2962-2968.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.135 synthesis panose migth be used as an anticariogenic sweetener in foods, development of a system for continuous production of extremely high panose syrup from pullulan by employing the enzyme Bacteroides thetaiotaomicron

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.135 additional information diverse mutants are constructed and specific activities, sugar compositions of pullulan hydrolysate and hydrolysis activities toward alpha-(1-4) and alpha-(1-6)-glucosidic linkages analyzed Bacteroides thetaiotaomicron

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.135 70000
-
 gel filtration Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.135 pullulan + H2O Bacteroides thetaiotaomicron
-
 panose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Bacteroides thetaiotaomicron
-
 95-1
-
3.2.1.20 Bacteroides thetaiotaomicron 95-1
-
 95-1
-
3.2.1.135 Bacteroides thetaiotaomicron
-
 95-1
-
3.2.1.135 Bacteroides thetaiotaomicron 95-1
-
 95-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 partial Bacteroides thetaiotaomicron
3.2.1.135
-
 Bacteroides thetaiotaomicron

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
-
 Bacteroides thetaiotaomicron
3.2.1.135 additional information
-
 comparison of wild-type and mutant enzymes Bacteroides thetaiotaomicron

Storage Stability

EC Number Storage Stability Organism
3.2.1.135 -20°C, 20% glycerol, stable Bacteroides thetaiotaomicron

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltotriose + H2O
-
 Bacteroides thetaiotaomicron alpha-D-glucose
-
 ?
3.2.1.20 isomaltotriose + H2O
-
 Bacteroides thetaiotaomicron 95-1 alpha-D-glucose
-
 ?
3.2.1.20 maltoheptaose + H2O
-
 Bacteroides thetaiotaomicron maltohexaose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O
-
 Bacteroides thetaiotaomicron maltopentaose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Bacteroides thetaiotaomicron alpha-D-glucose + ?
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Bacteroides thetaiotaomicron 95-1 alpha-D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Bacteroides thetaiotaomicron alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 Bacteroides thetaiotaomicron 95-1 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Bacteroides thetaiotaomicron alpha-D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Bacteroides thetaiotaomicron 95-1 alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Bacteroides thetaiotaomicron maltose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 Bacteroides thetaiotaomicron 95-1 maltose + D-glucose
-
 ?
3.2.1.20 panose + H2O
-
 Bacteroides thetaiotaomicron maltose + alpha-D-glucose
-
 ?
3.2.1.135 maltoheptaose + H2O
-
 Bacteroides thetaiotaomicron D-glucose + maltose + ?
-
 ?
3.2.1.135 maltohexaose + H2O
-
 Bacteroides thetaiotaomicron D-glucose + maltose + ?
-
 ?
3.2.1.135 maltopentaose + H2O
-
 Bacteroides thetaiotaomicron D-glucose + maltose + ?
-
 ?
3.2.1.135 maltotetraose + H2O
-
 Bacteroides thetaiotaomicron 2 D-glucose + maltose
-
 ?
3.2.1.135 maltotriose + H2O
-
 Bacteroides thetaiotaomicron maltose + D-glucose
-
 ?
3.2.1.135 additional information enzyme is able to cleave beta-cyclodextrin Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.135 additional information enzyme also cleaves alpha-1,4-bonds in amylose and in oligosaccharides of maltotriose through maltoheptaose in chain length Bacteroides thetaiotaomicron ?
-
 ?
3.2.1.135 pullulan + H2O
-
 Bacteroides thetaiotaomicron panose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.135 monomer 1 * 70000, SDS-PAGE Bacteroides thetaiotaomicron