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Literature summary extracted from

  • Saha, B.C.; Zeikus, J.G.
    Characterization of thermostable alpha-glucosidase from Clostridium thermosulfuricum 39E (1991), Appl. Microbiol. Biotechnol., 35, 568-571.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 acarbose
-
Thermoanaerobacter thermohydrosulfuricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.31
-
maltotriose
-
Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 0.58
-
maltotriose
-
Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 1.72
-
panose
-
Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 1.85
-
maltose
-
Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 2.95
-
isomaltose
-
Thermoanaerobacter thermohydrosulfuricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Thermoanaerobacter thermohydrosulfuricus
-
39E
-
3.2.1.20 Thermoanaerobacter thermohydrosulfuricus 39E
-
39E
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 partial Thermoanaerobacter thermohydrosulfuricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 5.2
-
-
Thermoanaerobacter thermohydrosulfuricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O
-
Thermoanaerobacter thermohydrosulfuricus 2 alpha-D-glucose
-
?
3.2.1.20 isomaltose + H2O
-
Thermoanaerobacter thermohydrosulfuricus 39E 2 alpha-D-glucose
-
?
3.2.1.20 isomaltotriose + H2O
-
Thermoanaerobacter thermohydrosulfuricus alpha-D-glucose
-
?
3.2.1.20 isomaltotriose + H2O
-
Thermoanaerobacter thermohydrosulfuricus 39E alpha-D-glucose
-
?
3.2.1.20 maltohexaose + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltopentaose + D-glucose
-
?
3.2.1.20 maltose + H2O
-
Thermoanaerobacter thermohydrosulfuricus alpha-D-glucose + D-glucose
-
?
3.2.1.20 maltose + H2O
-
Thermoanaerobacter thermohydrosulfuricus 39E alpha-D-glucose + D-glucose
-
?
3.2.1.20 maltotetraose + H2O
-
Thermoanaerobacter thermohydrosulfuricus alpha-D-glucose
-
?
3.2.1.20 maltotetraose + H2O
-
Thermoanaerobacter thermohydrosulfuricus 39E alpha-D-glucose
-
?
3.2.1.20 maltotriose + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltose + D-glucose
-
?
3.2.1.20 maltotriose + H2O
-
Thermoanaerobacter thermohydrosulfuricus 39E maltose + D-glucose
-
?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O
-
Thermoanaerobacter thermohydrosulfuricus 4-nitrophenol + D-glucose
-
?
3.2.1.20 panose + H2O
-
Thermoanaerobacter thermohydrosulfuricus maltose + alpha-D-glucose
-
?
3.2.1.20 phenyl alpha-D-glucoside + H2O
-
Thermoanaerobacter thermohydrosulfuricus phenol + alpha-D-glucose
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 75
-
-
Thermoanaerobacter thermohydrosulfuricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 48 80 48°C: about 50% of maximal activity, 80°C: about 95% of maximal activity Thermoanaerobacter thermohydrosulfuricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 60
-
half-life: 46 h Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 70
-
half-life: 110 min Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 75
-
half-life: 35 min Thermoanaerobacter thermohydrosulfuricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5 5.5
-
Thermoanaerobacter thermohydrosulfuricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 4.5 6 pH 4.5: 90% of maximal activity, pH 6.0: 94% of maximal activity Thermoanaerobacter thermohydrosulfuricus
3.2.1.20 5 5.5
-
Thermoanaerobacter thermohydrosulfuricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 5 6 most stable at Thermoanaerobacter thermohydrosulfuricus