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Literature summary extracted from

  • Li, K.B.; Chan, K.Y.
    Production and properties of alpha-glucosidase from Lactobacillus acidophilus (1983), Appl. Environ. Microbiol., 46, 1380-1387.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.20 EDTA stimulates Lactobacillus acidophilus
3.2.1.20 L-Cys stimulates Lactobacillus acidophilus
3.2.1.20 L-His stimulates Lactobacillus acidophilus
3.2.1.20 Tris stimulates Lactobacillus acidophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 2-Iodoacetamide
-
 Lactobacillus acidophilus
3.2.1.20 Ca2+
-
 Lactobacillus acidophilus
3.2.1.20 Co2+
-
 Lactobacillus acidophilus
3.2.1.20 Cu2+
-
 Lactobacillus acidophilus
3.2.1.20 Hg2+
-
 Lactobacillus acidophilus
3.2.1.20 Ni2+
-
 Lactobacillus acidophilus
3.2.1.20 Rose bengal
-
 Lactobacillus acidophilus
3.2.1.20 Urea 4.5 M, 22 min, 40% inhibition Lactobacillus acidophilus
3.2.1.20 Zn2+
-
 Lactobacillus acidophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 11.5
-
 4-nitrophenyl beta-D-glucopyranoside
-
 Lactobacillus acidophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 cytoplasm
-
 Lactobacillus acidophilus 5737
-
3.2.1.20 intracellular
-
 Lactobacillus acidophilus 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 Mg2+ stimulates Lactobacillus acidophilus
3.2.1.20 Mn2+ stimulates Lactobacillus acidophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Lactobacillus acidophilus
-
 IFO 3532
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 Lactobacillus acidophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 1451
-
-
 Lactobacillus acidophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Lactobacillus acidophilus 4-nitrophenol + beta-D-glucopyranose
-
 ?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O p-nitrophenyl-alpha-D-glucopyranoside Lactobacillus acidophilus 4-nitrophenol + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 37
-
-
 Lactobacillus acidophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 20 50 20°C: about 50% of maximal activity, 50°C: about 60% of maximal activity Lactobacillus acidophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5
-
-
 Lactobacillus acidophilus