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Literature summary extracted from

  • Giblin, M.; Kelly, C.T.; Fogarty, W.M.
    Thermostable alpha-glucosidase produced by Bacillus caldovelox DSM411 (1987), Can. J. Microbiol., 33, 614-618.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.20 2,2'-dipyridyl 1 mM, about 6.5fold activation [Bacillus] caldovelox
3.2.1.20 D-glucose activates [Bacillus] caldovelox
3.2.1.20 EDTA 1 mM, about 4.5fold activation [Bacillus] caldovelox
3.2.1.20 p-phenanthroline 1 mM, about 8fold activation [Bacillus] caldovelox

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Ba2+ 10 mM, activates [Bacillus] caldovelox
3.2.1.20 D-glucono-delta-lactone
-
 [Bacillus] caldovelox
3.2.1.20 phenyl-alpha-D-glucoside
-
 [Bacillus] caldovelox
3.2.1.20 ribose
-
 [Bacillus] caldovelox
3.2.1.20 Tris
-
 [Bacillus] caldovelox

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 intracellular
-
 [Bacillus] caldovelox 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 Mn2+ 1 mM, about 4.5fold activation [Bacillus] caldovelox
3.2.1.20 Sr2+ 10 mM, 3fold activation [Bacillus] caldovelox

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 [Bacillus] caldovelox
-
 DSM411
-
3.2.1.20 [Bacillus] caldovelox DSM 411
-
 DSM411
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
 [Bacillus] caldovelox

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 71.4
-
-
 [Bacillus] caldovelox

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 isomaltose + H2O no activity [Bacillus] caldovelox 2 alpha-D-glucose
-
 ?
3.2.1.20 isomaltose + H2O no activity [Bacillus] caldovelox DSM 411 2 alpha-D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 [Bacillus] caldovelox alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltose + H2O
-
 [Bacillus] caldovelox DSM 411 alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 [Bacillus] caldovelox maltose + D-glucose
-
 ?
3.2.1.20 maltotriose + H2O
-
 [Bacillus] caldovelox DSM 411 maltose + D-glucose
-
 ?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O best substrate [Bacillus] caldovelox 4-nitrophenol + D-glucose
-
 ?
3.2.1.20 p-nitrophenyl-alpha-D-glucopyranoside + H2O best substrate [Bacillus] caldovelox DSM 411 4-nitrophenol + D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 50 60
-
 [Bacillus] caldovelox

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 20 70 about 35% of maximal activity at 20°C and at 70°C [Bacillus] caldovelox

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 60
-
 1 h, stable [Bacillus] caldovelox
3.2.1.20 70
-
 1 h, 50% loss of activity. Retains 83% or more activity after 1.5 h in presence of Mn2+, Cys, and His [Bacillus] caldovelox

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5 6
-
 [Bacillus] caldovelox

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 4 8 pH 4.0: about 10% of maximal activity, pH 8.0: about 55% of maximal activity [Bacillus] caldovelox

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 4 9 20 min, retains more than 80% of its activity [Bacillus] caldovelox
3.2.1.20 5.5 6.5 20 min, completely stable [Bacillus] caldovelox