EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | additional information | omission of the component A, B or C, as well as methyl coenzyme M, H2, Mg2+ or ATP leads to complete loss of activity and methane biosynthesis | Methanothermobacter thermautotrophicus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | CO | little blocking effect on the hydrogenase function of component A | Methanothermobacter thermautotrophicus | |
2.8.4.1 | O2 | total loss of activity of hydrogenase activity of component A | Methanothermobacter thermautotrophicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.8.4.1 | Mg2+ | - |
Methanothermobacter thermautotrophicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | additional information | - |
- |
Methanothermobacter thermautotrophicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.8.4.1 | Methanobacterium bryantii | - |
M.o.H. and M.o.H.G. | - |
2.8.4.1 | Methanobacterium formicicum | - |
- |
- |
2.8.4.1 | Methanosarcina barkeri | - |
- |
- |
2.8.4.1 | Methanospirillum hungatei | - |
- |
- |
2.8.4.1 | Methanothermobacter thermautotrophicus | - |
- |
- |
2.8.4.1 | no activity in Methanobrevibacter ruminantium | - |
- |
- |
2.8.4.1 | no activity in Methanobrevibacter ruminantium M-1 | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
2.8.4.1 | oxygen causes irreversible loss of methane forming activity, component C is oxygen-stable | Methanothermobacter thermautotrophicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.8.4.1 | anaerobic conditions, ion-exchange column, gel-filtration | Methanothermobacter thermautotrophicus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 0.00046 | - |
- |
Methanosarcina barkeri |
2.8.4.1 | 0.000535 | - |
- |
Methanobacterium bryantii |
2.8.4.1 | 0.00054 | - |
- |
Methanothermobacter thermautotrophicus |
2.8.4.1 | 0.000935 | - |
- |
Methanobacterium formicicum |
2.8.4.1 | 0.00094 | - |
- |
Methanobacterium bryantii |
2.8.4.1 | 0.001135 | - |
- |
Methanospirillum hungatei |
EC Number | Storage Stability | Organism |
---|---|---|
2.8.4.1 | ice bath temperature, hydrogenase stable 3-4 weeks | Methanothermobacter thermautotrophicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.8.4.1 | methyl-coenzyme M + coenzyme B | in presence of H2 as source of electrons, requires Mg2+ and catalytic ATP | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
2.8.4.1 | additional information | ability of the hydrogenase to reduce a number of artificial and naturally occurring electron acceptors is examined | Methanothermobacter thermautotrophicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.8.4.1 | More | three components: A 500000 Da hydrogenase activity for reduction of viologen dyes, coenzyme F-420 or flavins, B 1000 Da oxygen labile and C 130000 Da, gel-filtration | Methanothermobacter thermautotrophicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 60 | - |
assay | Methanothermobacter thermautotrophicus |
2.8.4.1 | 60 | - |
for hydrogenase of component A | Methanothermobacter thermautotrophicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 95 | - |
10 min under anaerobic conditions, heat-labile components A and C lose activity | Methanothermobacter thermautotrophicus |
2.8.4.1 | 121 | - |
1-2 h, heat-stable component B, no loss of activity | Methanothermobacter thermautotrophicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.8.4.1 | 6 | - |
assay at | Methanothermobacter thermautotrophicus |
2.8.4.1 | 7 | - |
for hydrogenase of component A | Methanothermobacter thermautotrophicus |