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Literature summary extracted from

  • Jablonski, P.E.; Ferry, J.G.
    Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila (1991), J. Bacteriol., 173, 2481-2487.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.8.4.1 ATP stimulates the reactivation Methanosarcina thermophila
2.8.4.1 reduced ferredoxin reactivation after purification Methanosarcina thermophila
2.8.4.1 Ti(III) citrate reactivation after isolation Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.1 0.0033
-
 methyl coenzyme M
-
 Methanosarcina thermophila
2.8.4.1 0.059
-
 coenzyme B
-
 Methanosarcina thermophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.4.1 additional information no activity found in membrane fraction Methanosarcina thermophila
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 33000
-
 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila
2.8.4.1 42000
-
 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila
2.8.4.1 69000
-
 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila
2.8.4.1 132000
-
 nondenaturing slab gel electrophoresis Methanosarcina thermophila
2.8.4.1 141000
-
 gel filtration Methanosarcina thermophila
2.8.4.1 144000
-
-
 Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B Methanosarcina thermophila
-
 methane + CoM-S-S-CoB
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanosarcina thermophila
-
 TM-1, acetate grown
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 sucrose density gradient cetrifugation, Q-Sepharose, Phenyl-Superose 5/5 column, anaerobic Methanosarcina thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.4.1 0.224
-
-
 Methanosarcina thermophila

Storage Stability

EC Number Storage Stability Organism
2.8.4.1 4°C, 11 h, fully active Methanosarcina thermophila
2.8.4.1 liquid N2, 50 mM potassium phosphate buffer, pH 7.0, 1 M potassium acetate, 10 mM 2-mercaptoethanol, 10% w/v glycerol Methanosarcina thermophila
2.8.4.1 three freeze-thaw cycles, fully active Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B
-
 Methanosarcina thermophila methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 trimer 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma Methanosarcina thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 60
-
-
 Methanosarcina thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.8.4.1 4
-
 11 h, fully active Methanosarcina thermophila
2.8.4.1 23
-
 N2 atmosphere, 2 h, 28% loss of original activity Methanosarcina thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 6.5 7
-
 Methanosarcina thermophila

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 1 mol coenzyme F-430/mol enzyme Methanosarcina thermophila