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Literature summary extracted from

  • Bonacker, L.G.; Baudner, S.; Morschel, E.; Bocher, R.; Thauer, R.K.
    Properties of the two isoenzymes of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1993), Eur. J. Biochem., 217, 587-595.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.8.4.1 activity is stabilized by high concentrations of ammonium sulfate Methanothermobacter thermautotrophicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.4.1 3-bromopropanesulfonate most potent inhibitor, competitive with respect to methyl-coenzyme M Methanothermobacter thermautotrophicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.4.1 0.1 0.3 coenzyme B isoenzyme I Methanothermobacter thermautotrophicus
2.8.4.1 0.4 0.6 coenzyme B isoenzyme II Methanothermobacter thermautotrophicus
2.8.4.1 0.6 0.8 methyl coenzyme M isoenzyme I Methanothermobacter thermautotrophicus
2.8.4.1 1.3 1.5 methyl coenzyme M isoenzyme II Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.4.1 cytoplasm
-
 Methanothermobacter thermautotrophicus 5737
-
2.8.4.1 membrane on the inner surface of the cytoplasmic membrane Methanothermobacter thermautotrophicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.8.4.1 Ni(2+) 2 mol Ni/mol enzyme Methanothermobacter thermautotrophicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.4.1 300000
-
-
 Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus rate limiting step in methanogenesis methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus energy metabolism of all methanogenic archaea methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 rate limiting step in methanogenesis methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B Methanothermobacter thermautotrophicus Marburg / DSM 2133 energy metabolism of all methanogenic archaea methane + CoM-S-S-CoB
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.4.1 Methanothermobacter thermautotrophicus
-
-
-
2.8.4.1 Methanothermobacter thermautotrophicus
-
 produces different isoenzymes under different growth conditions
-
2.8.4.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.8.4.1 high concentrations of both H2 and CO2 favour the expression of isoenzyme II, low concentrations favour the expression of isoenzyme I Methanothermobacter thermautotrophicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.4.1 copurification of isoenzymes, separation of isoenzymes by anion-exchange chromatography Methanothermobacter thermautotrophicus
2.8.4.1 hydrophobic interaction chromatography, Sepharose beads which contain antibodies specific for each isoenzyme, immunoadsorption method Methanothermobacter thermautotrophicus
2.8.4.1 under 5% H2/95% N2 condition, fractionated ammonium sulfate precipitation Methanothermobacter thermautotrophicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.8.4.1 methyl-CoM + CoB = CoM-S-S-CoB + methane ternary complex type Methanothermobacter thermautotrophicus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.4.1 4.5
-
 isoenzyme I Methanothermobacter thermautotrophicus
2.8.4.1 11.7
-
 isoenzyme II Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
2.8.4.1 -70°C, rapidly frozen, yellow precipitate, suspended in 100%-saturated ammonium sulfate, stored unter N2, remains active Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.4.1 methyl coenzyme M + coenzyme B rate limiting step in methanogenesis Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B energy metabolism of all methanogenic archaea Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B rate limiting step in methanogenesis Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl coenzyme M + coenzyme B energy metabolism of all methanogenic archaea Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus methane + CoM-S-S-CoB
-
 ?
2.8.4.1 methyl-coenzyme M + coenzyme B
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 methane + CoM-S-S-CoB
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.4.1 hexamer alpha2,beta2,gamma2 Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.4.1 65
-
 assay at Methanothermobacter thermautotrophicus
2.8.4.1 68 70 both isoenzymes Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.4.1 7 7.5 isoenzyme I Methanothermobacter thermautotrophicus
2.8.4.1 7
-
 assay at Methanothermobacter thermautotrophicus
2.8.4.1 7.5 8 isoenzyme II Methanothermobacter thermautotrophicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.8.4.1 F-430 nickel porphinoid coenzyme M Methanothermobacter thermautotrophicus
2.8.4.1 F-430 2 mol coenzyme F-430/mol enzyme Methanothermobacter thermautotrophicus
2.8.4.1 F-430 2 mol nickel porphinoid/mol enzyme, prosthetic group Methanothermobacter thermautotrophicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.4.1 0.0001
-
 3-bromopropanesulfonate for both isoenzymes Methanothermobacter thermautotrophicus