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Literature summary extracted from

  • Richard, S.B.; Bowman, M.E.; Kwiatkowski, W.; Kang, L.; Chow, C.; Lillo, A.M.; Cane, D.E.; Noel, J.P.
    Structure of 4-diphosphocytidyl-2-C-methylerythritol synthetase involved in mevalonate-independent isoprenoid biosynthesis (2001), Nature Struct. Biol., 8, 641-648.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.60 expressed in Escherichia coli BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.60 vapor diffusion method at 4°C in hanging drops, crystals belong to space group C2 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.60 K213S effective turnover rate is ca. 0.4% of the rate of wild type, retains capacity to form product with significantly reduced efficiency Escherichia coli
2.7.7.60 K27A amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme Escherichia coli
2.7.7.60 K27S amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.60 Mg2+ essential for activity Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Mycobacterium tuberculosis
-
 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Plasmodium falciparum
-
 diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli third step of biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate Escherichia coli part of mevalonate-indpendent pathway for isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.60 Escherichia coli
-
-
-
2.7.7.60 Mycobacterium tuberculosis
-
-
-
2.7.7.60 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.60 Ni2+-nitrilotriacetic acid column Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol cytidyltransferase family. Active substrate site consists of a glycine-rich loop spanning Pro13-Arg20. Three-dimensional structure of enzyme is figured out Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate
-
 Mycobacterium tuberculosis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate
-
 Plasmodium falciparum diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate third step of biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol-4-phosphate part of mevalonate-indpendent pathway for isoprenoid biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.60 dimer homodimer Escherichia coli
2.7.7.60 dimer each subunit contains a globular core domain with an alpha/beta sturcture and and one smaller subdomain Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.60 25
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.60 8
-
 assay at Escherichia coli