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Literature summary extracted from

  • Siegel, L.M.; Davis, P.S.; Kamin, H.
    Reduced nicotinamide adenine dinucleotide phosphate-sulfite reductase of enterobacteria. 3. The Escherichia coli hemoflavoprotein: catalytic parameters and the sequence of electron flow (1974), J. Biol. Chem., 249, 1572-1586.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.2 arsenite
-
 Escherichia coli
1.8.1.2 Br-
-
 Escherichia coli
1.8.1.2 Cl-
-
 Escherichia coli
1.8.1.2 CN-
-
 Escherichia coli
1.8.1.2 CO
-
 Escherichia coli
1.8.1.2 F-
-
 Escherichia coli
1.8.1.2 NADP+ NADPH-dependent activities Escherichia coli
1.8.1.2 NO3-
-
 Escherichia coli
1.8.1.2 p-Mercuriphenylsulfonate
-
 Escherichia coli
1.8.1.2 SCN-
-
 Escherichia coli
1.8.1.2 sulfate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.2 0.0043 0.0074 sulfite
-
 Escherichia coli
1.8.1.2 0.8 1.5 nitrite
-
 Escherichia coli
1.8.1.2 4.5 10.5 hydroxylamine
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.2 sulfite + NADPH + H+ Escherichia coli no physiological nitrite reductase sulfide + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.2 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.1.2 hydrogen sulfide + 3 NADP+ + 3 H2O = sulfite + 3 NADPH + 3 H+ mechanism of electron transfer Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.2 hydroxylamine + NADPH NADPH can be replaced by reduced methyl viologen Escherichia coli ammonia + NADP+
-
 ?
1.8.1.2 additional information transfer of hydrogen from NADPH to 3-acetylpyridineadenine dinucleotide phosphate Escherichia coli ?
-
 ?
1.8.1.2 additional information reactions catalyzed by flavoprotein subunit alone: transfer of electrons from NADPH to cytochrome c, ferricyanide, dichlorphenolindophenol, menadione, FMN, FAD, O2 Escherichia coli ?
-
 ?
1.8.1.2 NADP+ + reduced methyl viologen
-
 Escherichia coli NADPH + oxidized methyl viologen
-
 ?
1.8.1.2 nitrite + NADPH
-
 Escherichia coli ammonia + NADP+
-
 ?
1.8.1.2 sulfite + NADPH NADPH can be replaced by reduced methyl viologen Escherichia coli sulfide + NADP+ + H2O
-
 ?
1.8.1.2 sulfite + NADPH + H+ no physiological nitrite reductase Escherichia coli sulfide + NADP+ + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.1.2 1.25
-
 NADPH cosubstrate O2 Escherichia coli
1.8.1.2 30 40 sulfite cosubstrate NADPH Escherichia coli
1.8.1.2 43.3 51.7 nitrite cosubstrate NADPH Escherichia coli
1.8.1.2 65
-
 sulfite cosubstrate reduced methyl viologen Escherichia coli
1.8.1.2 98.3 217 hydroxylamine cosubstrate NADPH Escherichia coli
1.8.1.2 158
-
 NADPH cosubstrate 3-acetylpyridineadenine dinucleotide phosphate Escherichia coli
1.8.1.2 172
-
 NADPH cosubstrate FMN Escherichia coli
1.8.1.2 318
-
 NADPH cosubstrate cytochrome c Escherichia coli
1.8.1.2 462
-
 NADPH cosubstrate dichlorphenolindophenol Escherichia coli
1.8.1.2 468
-
 NADPH cosubstrate ferricyanide Escherichia coli
1.8.1.2 605
-
 NADP+
-
 Escherichia coli
1.8.1.2 605
-
 reduced methyl viologen
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.2 7.9
-
 sulfite Escherichia coli
1.8.1.2 8.6
-
 nitrite Escherichia coli
1.8.1.2 9.5
-
 hydroxylamine Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.2 flavin treatment with p-chloromercuriphenylsulfonate causes dissociation of FMN but retetion of FAD and heme Escherichia coli