EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.4 | 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate | preincubation with NO2- completely protects | Derxia gummosa | |
1.7.1.4 | 2,2'-bipyridine | 1 mM, 40% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor | Derxia gummosa | |
1.7.1.4 | 8-hydroxyquinoline | 1 mM, 96% inhibition | Derxia gummosa | |
1.7.1.4 | antimycin A | 0.2 mM, 45% inhibition | Derxia gummosa | |
1.7.1.4 | CN- | 1 mM, 45% inhibition, no inhibition if enzyme is preincubated with nitrite or NH2OH prior to adding the inhibitor | Derxia gummosa | |
1.7.1.4 | dinitrophenol | - |
Derxia gummosa | |
1.7.1.4 | hydroxylamine | competitive vs. nitrite | Derxia gummosa | |
1.7.1.4 | Iodine | - |
Derxia gummosa | |
1.7.1.4 | KCNS | 0.1 mM, 90% inhibition of NH2OH reductase activity, nitrite reductase activity is not affected | Derxia gummosa | |
1.7.1.4 | N-ethylmaleimide | 0.1 mM, 52% inhibition | Derxia gummosa | |
1.7.1.4 | NH2OH | competitive vs. NO2- | Derxia gummosa | |
1.7.1.4 | nitrite | competitive vs. hydroxylamine | Derxia gummosa | |
1.7.1.4 | p-chloromercuribenzoate | 0.1 mM, 60% inhibition | Derxia gummosa | |
1.7.1.4 | Rose bengal | preincubation with NO2- completely protects | Derxia gummosa | |
1.7.1.4 | rotenone | 0.1 mM, 56% inhibition | Derxia gummosa | |
1.7.1.10 | 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate | 1.5 mM, 68% inhibition, hydroxylamine protects | Derxia gummosa | |
1.7.1.10 | 2,2'-dipyridyl | - |
Derxia gummosa | |
1.7.1.10 | 8-hydroxyquinoline | 0.1 mM, 50% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
1.7.1.10 | Antimycin 1 | 0.1 mM, 26% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
1.7.1.10 | dinitrophenol | 0.1 mM, 80% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
1.7.1.10 | Iodine | 0.5 mM, 80% inhibition, preincubation with hydroxylamine protects | Derxia gummosa | |
1.7.1.10 | N-ethylmaleimide | 0.1 mM, 58% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
1.7.1.10 | NADH | inhibits in absence of hydroxylamine | Derxia gummosa | |
1.7.1.10 | nitrite | nitrite and hydroxylamine reductase activity are associated with the same enzyme, nitrite and hydroxylamine are competitive for the same binding site | Derxia gummosa | |
1.7.1.10 | p-chloromercuribenzoate | 0.1 mM, 67% inhibition of hydroxylamine reductase activity | Derxia gummosa | |
1.7.1.10 | potassium cyanide | - |
Derxia gummosa | |
1.7.1.10 | potassium thiocyanate | 0.1 mM, 90% inhibition of hydroxylamine reductase activity, nitrite reductase activity is not inhibited | Derxia gummosa | |
1.7.1.10 | Rose bengal | 0.5 mM, 94% inhibition | Derxia gummosa | |
1.7.1.10 | rotenone | 0.1 mM, 52% inhibition of hydroxylamine reductase activity | Derxia gummosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.1.4 | 0.0048 | - |
NO2- | - |
Derxia gummosa | |
1.7.1.4 | 0.0063 | - |
NADH | + NO2- | Derxia gummosa | |
1.7.1.4 | 0.15 | - |
NADH | + hydroxylamine | Derxia gummosa | |
1.7.1.4 | 5.3 | - |
hydroxylamine | - |
Derxia gummosa | |
1.7.1.10 | 0.0048 | - |
NO2- | - |
Derxia gummosa | |
1.7.1.10 | 0.15 | - |
NADH | - |
Derxia gummosa | |
1.7.1.10 | 5.3 | - |
hydroxylamine | - |
Derxia gummosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.4 | Derxia gummosa | - |
- |
- |
1.7.1.10 | Derxia gummosa | - |
nitrite and hydroxylamine reductase are associated within the same enzyme | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.1.10 | nitrite and hydroxylamine reductase are associated with the same enzyme | Derxia gummosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.1.4 | 0.422 | - |
reduction of NO2- | Derxia gummosa |
1.7.1.4 | 0.868 | - |
reduction of hydroxylamine | Derxia gummosa |
1.7.1.10 | 0.422 | - |
reduction of nitrite | Derxia gummosa |
1.7.1.10 | 0.68 | - |
reduction of hydroxylamine | Derxia gummosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.4 | NAD(P)H + NH2OH | - |
Derxia gummosa | NAD(P)+ + NH3 + H2O | - |
? | |
1.7.1.4 | NAD(P)H + nitrite + H+ | NADH is preferred, reduced benzyl viologen or methyl viologen can act as artificial electron donors | Derxia gummosa | NAD(P)+ + NH3 + H2O | - |
? | |
1.7.1.4 | nitrite + NADH + H+ | - |
Derxia gummosa | NH3 + NAD+ + H2O | - |
r | |
1.7.1.10 | hydroxylamine + NADH | 100% activity with NADH, 46% with NADPH, 117% with FADH2, 105% with FMNH2, 220% with methylviologen, 210% with benzyl viologen, 57% with Na2S2O4 | Derxia gummosa | NH3 + NAD+ + H2O | - |
? | |
1.7.1.10 | NO2- + NADH | enzyme has both nitrite reductase and hydroxylamine reductase activity, main function may be nitrite reduction | Derxia gummosa | NH3 + H2O + NAD+ | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.1.4 | 8.5 | - |
- |
Derxia gummosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.1.4 | NADH | - |
Derxia gummosa | |
1.7.1.4 | NADPH | - |
Derxia gummosa | |
1.7.1.10 | NADH | - |
Derxia gummosa |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.1.4 | 0.52 | - |
NO2- | - |
Derxia gummosa | |
1.7.1.4 | 24 | - |
NH2OH | - |
Derxia gummosa | |
1.7.1.10 | 0.52 | - |
NO2- | - |
Derxia gummosa |