Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Quinn, G.B.; Trimboli, A.J.; Prosser, I.M.; Barber, M.J.
    Spectroscopic and kinetic properties of a recombinant form of the flavin domain of spinach NADH:nitrate reductase (1996), Arch. Biochem. Biophys., 327, 151-160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.1 the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.1 adenine inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 adenosine inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 adenosine 5'-diphosphoribose inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 ADP inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 AMP inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 ferricyanide inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 NAD+ inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 nicotinamide inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 NMN inhibition of the recombinant FAD domain Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.1 additional information
-
 additional information Km-values of the the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli Spinacia oleracea
1.7.1.1 0.017
-
 NADH recombinant FAD domain Spinacia oleracea
1.7.1.1 0.034
-
 ferricyanide recombinant FAD domain Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.1 additional information
-
 the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in E. coli show a MW of 30000 Da by SDS-PAGE Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1 the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 additional information the isolated flavin-containing domain is capable of reducing cytochrome b5 directly Spinacia oleracea ?
-
 ?
1.7.1.1 nitrate + NADH
-
 Spinacia oleracea nitrite + NAD+ + H2O
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.1 6.5
-
-
 Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.1.1 0.2
-
 ADP inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 0.2
-
 ADPribose inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 0.6
-
 AMP inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 1.9
-
 NAD+ inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 4.8
-
 adenosine inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 23
-
 ferricyanide inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 26
-
 adenine inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 62
-
 NMN inhibition of the recombinant FAD domain Spinacia oleracea
1.7.1.1 122
-
 nicotinamide inhibition of the recombinant FAD domain Spinacia oleracea