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Literature summary extracted from

  • De La Rosa, F.F.; Castillo, F.; Palacian, E.
    Effects of denaturing agents on spinach nitrate reductase (1977), Phytochemistry, 16, 875-879.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.7.1.1 0.03% SDS produces almost complete inactivation of NADH-diaphorase and NADH-nitrate reductase, while FNH2-nitrate reductase retains 60% of the original activity, FAD has no protecting effect Spinacia oleracea
1.7.1.1 1 M guanidine hydrochloride, in presence of FAD, 80% inactivation of FNH2-nitrate reductase activity and NADH-nitrate reductase activity, NADH-diaphorase activity is unaffected Spinacia oleracea
1.7.1.1 4 M urea, in presence of FAD, inactivation of FMNH2-nitrate reductase and NADH-nitrate reductase activity, only a slight effect on the NADH-diaphorase activity Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.1 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.1
-
 Spinacia oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.1 additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.1 FMNH2 + nitrate
-
 Spinacia oleracea FMN + nitrite
-
 ?
1.7.1.1 additional information NADH-diaphorase activity Spinacia oleracea ?
-
 ?
1.7.1.1 nitrate + NADH
-
 Spinacia oleracea nitrite + NAD+ + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.1 25
-
 20 min, in absence of FAD, stable Spinacia oleracea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.1 6.5 8.5 1 h, stable Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.1 NADH
-
 Spinacia oleracea