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Literature summary extracted from

  • Kitajima, S.; Yasukochi, Y.; Minakami, S.
    Purification and properties of human erythrocyte membrane NADH-cytochrome b5 reductase (1981), Arch. Biochem. Biophys., 210, 330-339.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.2.2 Acrynol
-
 Homo sapiens
1.6.2.2 adenine nucleotides
-
 Homo sapiens
1.6.2.2 ADP
-
 Homo sapiens
1.6.2.2 Atebrin
-
 Homo sapiens
1.6.2.2 N-ethylmaleimide
-
 Homo sapiens
1.6.2.2 p-chloromercuribenzoate
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.2 0.0006
-
 NADH enzyme from erythrocyte membrane Homo sapiens
1.6.2.2 0.02
-
 ferricytochrome b5 enzyme from erythrocyte membrane Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.2 cytosol
-
 Homo sapiens 5829
-
1.6.2.2 membrane
-
 Homo sapiens 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.2 erythrocyte soluble form Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.2 790
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.2 2 ferricytochrome b5 + NADH specific for NADH as electron donor, artificial acceptors: ferricyanide, 2,6-dichlorphenolindophenol Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 2 ferricytochrome b5 + NADH additional acceptor: methemoglobin-ferrocyanide complex Homo sapiens 2 ferrocytochrome b5 + NAD+ + H+
-
 ?
1.6.2.2 NADH + ferricytochrome b5
-
 Homo sapiens NAD+ + H+ + ferrocytochrome b5
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.2 5.2
-
 NADH-methemoglobin-ferrocyanide Homo sapiens
1.6.2.2 6.5 8.5 NADH-ferricyanide Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.6.2.2 5 8 depending on acceptor Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.2 FAD
-
 Homo sapiens
1.6.2.2 NADH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.2.2 0.08
-
 ADP
-
 Homo sapiens