EC Number | General Stability | Organism |
---|---|---|
1.7.2.3 | about 50% of the activity of the molybdoenzyme is lost in the presence of 0.1 M NaCl, only about 4% of its initial activity is left in the presence of 2 M NaCl, 15% of the initial activity of the tungsten-substituted enzyme is left at 2 M NaCl | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.2.3 | 0.00754 | - |
4-methylmorpholine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.00765 | - |
Trimethylamine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.00838 | - |
gamma-picoline N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.00991 | - |
alpha-picoline N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.0108 | - |
Pyridine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.0252 | - |
tetramethylene sulfoxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.0278 | - |
Trimethylamine N-oxide | wild-type enzyme | Escherichia coli | |
1.7.2.3 | 0.0278 | - |
diphenylsulfoxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.0316 | - |
Trimethylamine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.0421 | - |
4-methylmorpholine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.631 | - |
gamma-picoline N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.653 | - |
alpha-picoline N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 0.974 | - |
Dimethylsulfoxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 2.25 | - |
Pyridine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 10.9 | - |
hydroxylamine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 11.8 | - |
hydroxylamine N-oxide | molybdenum-restored enzyme | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.3 | Molybdenum | in vivo tungsten substitution of the molybdoenzyme, 0.89 atoms of tungsten per mol of purified enzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.2.3 | Escherichia coli | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.2.3 | 41 | - |
purified tungsten-substituted enzyme | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.3 | 4-methylmorpholine N-oxide + electron donor | wild-type enzyme, molybdenum-restored enzyme and tungsten-restored enzyme | Escherichia coli | 4-methylmorpholine + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | alpha-picoline N-oxide + electron donor | wild-type enzyme, molybdenum-restored enzyme and tungsten-restored enzyme | Escherichia coli | alpha-picoline + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | dimethylsulfoxide + electron donor | tungsten-substituted enzyme | Escherichia coli | ? + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | diphenylsulfoxide + electron donor | tungsten-substituted enzyme | Escherichia coli | diphenylsulfide + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | gamma-picoline N-oxide + electron donor | wild-type enzyme, molybdenum-restored enzyme and tungsten-restored enzyme | Escherichia coli | gamma-picoline + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | hydroxylamine N-oxide + electron donor | wild-type enzyme, molybdenum-restored enzyme and tungsten-restored enzyme | Escherichia coli | hydroxylamine + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | pyridine N-oxide + electron donor | wild-type enzyme, molybdenum-restored enzyme and tungsten-restored enzyme | Escherichia coli | pyridine + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | tetramethylene sulfoxide + electron donor | tungsten-substituted enzyme | Escherichia coli | tetrahydrothiophene + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | trimethylamine N-oxide + electron donor | wild-type enzyme, molybdenum-restored enzyme and tungsten-restored enzyme | Escherichia coli | trimethylamine + oxidized electron donor + H2O | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.2.3 | 60 | - |
for molybdoenzyme, maximal activity remains the same at 80°C | Escherichia coli |
1.7.2.3 | 80 | - |
for tungsten-substituted enzyme, maximal level above | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.7.2.3 | 80 | - |
molybdoenzyme, after 4 min: 50% loss of activity, after 90 min: 97% loss of activity | Escherichia coli |
1.7.2.3 | 80 | - |
tungsten-substituted enzyme, after 90 min: more than 50% activity remains | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.2.3 | 26.8 | - |
diphenylsulfoxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 40.2 | - |
Dimethylsulfoxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 42.2 | - |
Pyridine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 43.6 | - |
hydroxylamine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 44.7 | - |
Pyridine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 52.2 | - |
4-methylmorpholine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 54.7 | - |
alpha-picoline N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 55.7 | - |
gamma-picoline N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 56 | - |
tetramethylene sulfoxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 57.2 | - |
gamma-picoline N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 58 | - |
Trimethylamine N-oxide | tungsten-restored enzyme | Escherichia coli | |
1.7.2.3 | 70.6 | - |
hydroxylamine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 71.4 | - |
4-methylmorpholine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 84.2 | - |
alpha-picoline N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 114 | - |
Trimethylamine N-oxide | molybdenum-restored enzyme | Escherichia coli | |
1.7.2.3 | 258 | - |
Trimethylamine N-oxide | wild-type enzyme | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.7.2.3 | 5 | - |
for tungsten-substituted enzyme, a significant decrease in activity is found at pH 5.5 | Escherichia coli |
1.7.2.3 | 5 | 5.5 | for molybdoenzyme | Escherichia coli |