EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.3 | 1,10-phenanthroline | slight | Shewanella sp. | |
1.7.2.3 | 2,2-dipyridyl | slight | Shewanella sp. | |
1.7.2.3 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Shewanella sp. | |
1.7.2.3 | iodoacetate | partial | Shewanella sp. | |
1.7.2.3 | KCN | partial | Shewanella sp. | |
1.7.2.3 | Na2MoO4 | - |
Shewanella sp. | |
1.7.2.3 | Na2WO4 | - |
Shewanella sp. | |
1.7.2.3 | p-chloromercuribenzoate | - |
Shewanella sp. | |
1.7.2.3 | Sodium chlorate | partial | Shewanella sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.2.3 | 0.02 | - |
Trimethylamine N-oxide | - |
Shewanella sp. | |
1.7.2.3 | 2.41 | - |
N,N-dimethyldodecylamine N-oxide | - |
Shewanella sp. | |
1.7.2.3 | 6.95 | - |
gamma-picoline N-oxide | - |
Shewanella sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.7.2.3 | periplasm | - |
Shewanella sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.3 | Molybdenum | enzyme contains 1.32 mol molybdenum per mol of enzyme | Shewanella sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.7.2.3 | 47000 | - |
2 MW forms, MW: 47000, SDS-PAGE in absence of reducing agents and MW: 84000, gel filtration, SDS-PAGE under reducing conditions | Shewanella sp. |
1.7.2.3 | 84000 | - |
2 MW forms, MW: 47000, SDS-PAGE in absence of reducing conditions and MW: 84000, gel filtration, SDS-PAGE under reducing conditions | Shewanella sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.2.3 | Shewanella sp. | - |
marine bacterium, 2 enzyme forms | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.2.3 | of 2 enzyme forms, using ammonium sulfate treatment, ion exchange buffer treatment, column chromatography on DEAE-Sepharose CL6B and hydroxyapatite and gel filtration on Sephacryl S-300 | Shewanella sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.7.2.3 | 211.5 | - |
- |
Shewanella sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.2.3 | adenosine N-oxide + electron donor | 40% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 35% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | adenosine + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | gamma-picoline N-oxide + electron donor | 11% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 12% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | gamma-picoline + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | hydroxylamine N-oxide + electron donor | 19% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 3% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | hydroxylamine + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | additional information | no activity with FADH2, NADH, NADPH and the reduced forms of methylene blue and N-methylphenazonium methosulphate | Shewanella sp. | ? | - |
? | |
1.7.2.3 | N,N-dimethyldodecylamine N-oxide + electron donor | 63% relative activity to trimethylamine N-oxide with the periplasmic enzyme and 41% relative activity to trimethylamine N-oxide with the purified enzyme | Shewanella sp. | N,N-dimethyldodecylamine + H2O + oxidized electron donor | - |
? | |
1.7.2.3 | trimethylamine N-oxide + electron donor | methyl viologen as electron donor | Shewanella sp. | trimethylamine + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | trimethylamine N-oxide + electron donor | reduced methylene blue, reduced N-methylphenazonium methosulfate as electron donors | Shewanella sp. | trimethylamine + oxidized electron donor + H2O | - |
? | |
1.7.2.3 | trimethylamine N-oxide + electron donor | benzyl viologen as electron donor | Shewanella sp. | trimethylamine + oxidized electron donor + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.7.2.3 | monomer | 1 * 84000, SDS-PAGE under reducing conditions | Shewanella sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.7.2.3 | FAD | stimulation | Shewanella sp. | |
1.7.2.3 | FMN | stimulation | Shewanella sp. |