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Literature summary extracted from

  • Jacobs, E.; Heriot, K.; Fisher, R.R.
    Resolution and reconstitution of Rhodospirillum rubrum pyridine dinucleotide transhydrogenase. II. Solubilization of the membrane-bound component (1977), Arch. Microbiol., 115, 151-156.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.6.1.2 lysolecithin aprrox. 0.3%, 150% activation of activity in chromatophore extracts Rhodospirillum rubrum

General Stability

EC Number General Stability Organism
1.6.1.2 solubilized membrane component is unstable loosing all activity when stored overnight at 4°C or -70°C, stable at -70°C in the presence of 0.025 mM NADP+ for several months, inactivation by refreezing after thawing Rhodospirillum rubrum
1.6.1.2 trypsinolysis is stimulated several fold by NADPH and NADP+, half-maximal stimulation with 0.001-0.002 mM NADPH and 0.002-0.003 mM NADP+, Mg2+ protects from NADP+ stimulated inactivation Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.1.2 chromatophore enzyme is compromised of a membrane-bound component and an easily dissociable soluble factor Rhodospirillum rubrum
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.2 Rhodospirillum rubrum
-
-
-

Storage Stability

EC Number Storage Stability Organism
1.6.1.2 -70°C or 4°C, Rhodospirillum rubrum membrane component, inactivation in 1 day, stabilization by NADP+ Rhodospirillum rubrum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.1.2 44
-
2 min, solubilized membrane component, 50% inactivation Rhodospirillum rubrum
1.6.1.2 48
-
2 min, membrane particles, 50% inactivation Rhodospirillum rubrum

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.2 NAD+
-
Rhodospirillum rubrum
1.6.1.2 NADH
-
Rhodospirillum rubrum
1.6.1.2 NADP+
-
Rhodospirillum rubrum
1.6.1.2 NADPH
-
Rhodospirillum rubrum