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Literature summary extracted from

  • Enander, K.; Rydström, J.
    Energy-linked nicotinamide nucleotide transhydrogenase. Kinetics and regulation of purified and reconstituted transhydrogenase from beef heart mitochondria (1982), J. Biol. Chem., 257, 14760-14766.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.1.2 2'-AMP noncompetitive vs. NAD+, competitive vs. NADPH Bos taurus
1.6.1.2 5'-AMP competitive vs. NAD+, noncompetitive vs. NADPH Bos taurus
1.6.1.2 cardiolipin noncompetitive vs. NAD+ and NADPH Bos taurus
1.6.1.2 NADH competitive vs. NAD+, noncompetitive vs. NADPH Bos taurus
1.6.1.2 NADP+ noncompetitive vs. NAD+, competitive vs. NADPH Bos taurus
1.6.1.2 palmitoyl-CoA competitive inhibition vs. NADPH Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.1.2 0.0017
-
 NADP+ cosubstrate NADH Bos taurus
1.6.1.2 0.01
-
 NADH cosubstrate NADP+ Bos taurus
1.6.1.2 0.02
-
 NADPH cosubstrate NAD+ Bos taurus
1.6.1.2 0.029
-
 NADPH + acetylpyridine adenine dinucleotide Bos taurus
1.6.1.2 0.125
-
 NAD+ cosubstrate NADPH Bos taurus
1.6.1.2 0.166
-
 acetylpyridine adenine dinucleotide cosubstrate NADPH Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.1.2 mitochondrial membrane
-
 Bos taurus 31966
-

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.2 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.6.1.2 NADPH + NAD+ = NADP+ + NADH proposed proton-pump mechanism Bos taurus
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.6.1.2 reconstitution into liposomes Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.1.2 heart
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.2 NADH + NADP+ the reaction is coupled to a transmembrane proton translocation from cytosol to mitochondria Bos taurus NAD+ + NADPH
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.2 NAD+
-
 Bos taurus
1.6.1.2 NADH
-
 Bos taurus
1.6.1.2 NADP+
-
 Bos taurus
1.6.1.2 NADPH
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.6.1.2 0.01
-
 palmitoyl-CoA
-
 Bos taurus