EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.6.1.1 | 2'-AMP | almost no effect | Azotobacter vinelandii |
EC Number | General Stability | Organism |
---|---|---|
1.6.1.1 | bovine serum albumin, 0.2%, stabilization of diluted solutions | Azotobacter vinelandii |
1.6.1.1 | urea, 5 min, 50% inactivation | Azotobacter vinelandii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.6.1.1 | deoxycholate | - |
Azotobacter vinelandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.6.1.1 | 0.0003 | - |
FAD | at 25°C | Azotobacter vinelandii | |
1.6.1.1 | 0.0025 | - |
FAD | at 0°C | Azotobacter vinelandii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.6.1.1 | EDTA | slight activating at low buffer concentrations | Azotobacter vinelandii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.6.1.1 | 30000000 | 50000000 | rod-like structure, light scattering | Azotobacter vinelandii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.6.1.1 | Azotobacter vinelandii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.6.1.1 | - |
Azotobacter vinelandii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.6.1.1 | 220 | 260 | - |
Azotobacter vinelandii |
EC Number | Storage Stability | Organism |
---|---|---|
1.6.1.1 | 4°C, 0.1 M phosphate buffer, pH 7.5, 1 mM EDTA, several months, no loss of activity, storage at -20°C yields a partly insoluble enzyme | Azotobacter vinelandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.6.1.1 | NAD(P)H + 2,6-dichlorophenolindophenol | - |
Azotobacter vinelandii | NAD(P)+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.6.1.1 | NAD(P)H + K4Fe(CN)6 | - |
Azotobacter vinelandii | NAD(P)+ + K3Fe(CN)6 | - |
? | |
1.6.1.1 | NADH + thio-NAD+ | - |
Azotobacter vinelandii | NAD+ + thio-NADH | - |
? | |
1.6.1.1 | NADH + thio-NADP+ | - |
Azotobacter vinelandii | NAD+ + thio-NADPH | - |
? | |
1.6.1.1 | NADP+ + NADH | diaphorase-type reactions with NAD(P)H, K3Fe(CN)6 and 2,6-dichlorophenol indophenol | Azotobacter vinelandii | NADPH + NAD+ | - |
? | |
1.6.1.1 | NADPH + thio-NAD+ + H+[side 1] | - |
Azotobacter vinelandii | NADP+ + thio-NADH + H+[side 2] | - |
? | |
1.6.1.1 | NADPH + thio-NADP+ + H+[side 1] | - |
Azotobacter vinelandii | NADP+ + thio-NADPH + H+[side 2] | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.6.1.1 | 50 | - |
1 h stable, inactivation is dramatically accelerated by NADH and NADPH, partial protection by NADP+ and FMN, almost full protection by FAD | Azotobacter vinelandii |
1.6.1.1 | 65 | - |
15 min, complete inactivation, protection by FAD | Azotobacter vinelandii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.6.1.1 | FAD | FAD dissociates from the enzyme by heat treatment at 100°C or by treatment with 5% trichloroacetic acid at 0°C | Azotobacter vinelandii |