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Literature summary extracted from
- Transhydrogenase activity in the marine bacterium Beneckea natriegens (1977), Biochim. Biophys. Acta, 480, 77-82.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.6.1.1 | 2'-AMP | - |
Vibrio natriegens |  |
| 1.6.1.1 | 5'-AMP | 5 mM, 92% inhibition of NADP+ reduction, complete inhibition of NAD+ reduction | Vibrio natriegens | |
| 1.6.1.1 | ADP | 5 mM, 94% inhibition of NADP+ reduction, complete inhibition of NAD+ reduction | Vibrio natriegens | |
| 1.6.1.1 | arsenate | complete inhibition of activity in either direction | Vibrio natriegens | |
| 1.6.1.1 | ATP | 5 mM, 81% inhibition of NADP+ reduction, complete inhibition of NAD+ reduction | Vibrio natriegens | |
| 1.6.1.1 | CTP | 5 mM, 86% inhibition of NADP+ reduction | Vibrio natriegens | |
| 1.6.1.1 | diphosphate | 5 mM, 91% inhibition of NADP+ reduction | Vibrio natriegens | |
| 1.6.1.1 | GTP | 5 mM, 89% inhibition of NADP+ reduction | Vibrio natriegens | |
| 1.6.1.1 | phosphate | 5 mM, complete inhibition of activity in either direction | Vibrio natriegens | |
| 1.6.1.1 | phosphoenolpyruvate | 87% inhibition of NADP+ reduction | Vibrio natriegens | |
| 1.6.1.1 | pyridoxal 5'-phosphate | 5 mM, 91% inhibition of NADP+ reduction | Vibrio natriegens | |
| 1.6.1.1 | TTP | 2 mM, 71% inhibition of NADP+ reduction | Vibrio natriegens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.6.1.1 | Vibrio natriegens | - |
formerly Pseudomonas natriegens | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.6.1.1 | partial | Vibrio natriegens |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.6.1.1 | -15°C, 50 mM Tris-HCl buffer, pH 7.5, no loss of activity in 12 weeks | Vibrio natriegens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.6.1.1 | NADP+ + NADH | reduction of NADP+ is preferred | Vibrio natriegens | NADPH + NAD+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.6.1.1 | 7 | - |
NADH formation | Vibrio natriegens |
| 1.6.1.1 | 9.6 | - |
NADPH formation | Vibrio natriegens |
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