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Literature summary extracted from

  • Voordouw, G.; van der Vies, S.M.; Eweg, J.K.; Veeger, C.; van Breemen, J.F.L.; van Bruggen, E.F.J.
    Pyridine nucleotide transhydrogenase from Azotobacter vinelandii. Improved purification, physical properties and subunit arrangement in purified polymers (1980), Eur. J. Biochem., 111, 347-355.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.6.1.1
-
 Azotobacter vinelandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.1.1 52000
-
 x * 52000, SDS-PAGE, immunoblot Azotobacter vinelandii
1.6.1.1 54000
-
 8 * 54000, four subunits are placed in a rhomb, a second tetramer is located staggered on top of the first one, deduced from amino acid analysis and electron micrography of purified enzyme Azotobacter vinelandii
1.6.1.3 421000
-
 gel filtration Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.1.3 NADPH + NAD+ Azotobacter vinelandii
-
 NADP+ + NADH
-
 ?
1.6.1.3 NADPH + NAD+ Azotobacter vinelandii ATCC 478
-
 NADP+ + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.1 Azotobacter vinelandii
-
-
-
1.6.1.3 Azotobacter vinelandii
-
-
-
1.6.1.3 Azotobacter vinelandii ATCC 478
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.1 affinity chromatography Azotobacter vinelandii
1.6.1.3 Ado-2',5'-P2-Sepharose 4B column chromatography and Sephadex 200 gel filtration Azotobacter vinelandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.1.1 575
-
-
 Azotobacter vinelandii
1.6.1.3 0.56
-
 cell-free extract, in 50 mM Tris/CI pH 7.5, temperature not specified in the publication Azotobacter vinelandii
1.6.1.3 575
-
 after 1027fold purification, in 50 mM Tris/CI pH 7.5, temperature not specified in the publication Azotobacter vinelandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.3 NADPH + NAD+
-
 Azotobacter vinelandii NADP+ + NADH
-
 ?
1.6.1.3 NADPH + NAD+
-
 Azotobacter vinelandii ATCC 478 NADP+ + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.1.1 octamer x * 52000, SDS-PAGE, immunoblot Azotobacter vinelandii
1.6.1.1 octamer 8 * 54000, four subunits are placed in a rhomb, a second tetramer is located staggered on top of the first one, deduced from amino acid analysis and electron micrography of purified enzyme Azotobacter vinelandii

Synonyms

EC Number Synonyms Comment Organism
1.6.1.3 pyridine nucleotide transhydrogenase
-
 Azotobacter vinelandii

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.3 flavin
-
 Azotobacter vinelandii
1.6.1.3 NADPH
-
 Azotobacter vinelandii