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Literature summary extracted from

  • Hochstein, L.L.; Dalton, B.P.
    The purification and properties of nicotine oxidase (1967), Biochim. Biophys. Acta, 139, 56-68.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.99.4 1,10-phenanthroline 47% inhibition at 2 mM Pseudarthrobacter oxydans
1.5.99.4 2,2'-dipyridyl 27% inhibition at 2 mM Pseudarthrobacter oxydans
1.5.99.4 Acriflavine 64% inhibition at 1 mM Pseudarthrobacter oxydans
1.5.99.4 brilliant cresyl blue concentration over 0.2 mM Pseudarthrobacter oxydans
1.5.99.4 Cu2+
-
Pseudarthrobacter oxydans
1.5.99.4 cyanide 95% inhibition at 1 mM potassium cyanide Pseudarthrobacter oxydans
1.5.99.4 Hg2+ 96% inhibition at 0.1 mM Pseudarthrobacter oxydans
1.5.99.4 iodoacetamide 23% inhibition at 10 mM Pseudarthrobacter oxydans
1.5.99.4 additional information riboflavin 5'-phosphate protects enzyme against acriflavine inhibition, FMN protects enzyme against acriflavine inhibition, FAD only partially effective, glutathione protects against p-chloromercuriphenylsulfonic inhibition Pseudarthrobacter oxydans
1.5.99.4 p-chloromercuriphenylsulfonic acid 62% inhibition at 1 mM Pseudarthrobacter oxydans
1.5.99.4 Quinacrine 49% inhibition at 0.1 mM Pseudarthrobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.99.4 0.008
-
brilliant cresyl blue
-
Pseudarthrobacter oxydans
1.5.99.4 0.027
-
methylene blue
-
Pseudarthrobacter oxydans
1.5.99.4 0.037
-
Nicotine
-
Pseudarthrobacter oxydans
1.5.99.4 0.49
-
menadione
-
Pseudarthrobacter oxydans
1.5.99.4 1
-
vitamin K5
-
Pseudarthrobacter oxydans
1.5.99.4 1.24
-
2,6-dichlorophenolindophenol
-
Pseudarthrobacter oxydans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.99.4 Cu2+ inhibitory Pseudarthrobacter oxydans

Organism

EC Number Organism UniProt Comment Textmining
1.5.99.4 Pseudarthrobacter oxydans
-
P-34 DSM 419
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.99.4 ammonium sulfate precipitation, heat denaturation, TEAE-cellulose chromatography Pseudarthrobacter oxydans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.99.4 29.2
-
-
Pseudarthrobacter oxydans

Storage Stability

EC Number Storage Stability Organism
1.5.99.4 -19°C, 1 month, without thawing and refreezing, without loss of activity Pseudarthrobacter oxydans
1.5.99.4 -19°C, cells, 4 months, without loss of activity Pseudarthrobacter oxydans
1.5.99.4 4°C, 2-3 days, no significant loss of activity Pseudarthrobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.99.4 (S)-nicotine + 2,6-dichlorophenolindophenol + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced 2,6-dichlorophenolindophenol
-
r
1.5.99.4 (S)-nicotine + brilliant cresyl blue + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced brilliant cresyl blue
-
r
1.5.99.4 (S)-nicotine + menadione + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + menadiol
-
r
1.5.99.4 (S)-nicotine + methylene blue + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced methylene blue
-
r
1.5.99.4 (S)-nicotine + vitamin K5 + H2O
-
Pseudarthrobacter oxydans (S)-6-hydroxynicotine + reduced vitamin K5
-
r
1.5.99.4 anabasine + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxyanabasine + reduced acceptor
-
?
1.5.99.4 myosmine + acceptor + H2O
-
Pseudarthrobacter oxydans ? + reduced acceptor
-
?
1.5.99.4 nicotine + acceptor + H2O brilliant cresyl blue, methylene blue, 5-hydroxy-1,4-naphthoquinone, 2,6-dichlorophenolindophenol, menadione and vitamin K5 used as electron acceptors Pseudarthrobacter oxydans 6-hydroxynicotine + reduced acceptor
-
?
1.5.99.4 nicotine-N-oxide + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxynicotine-N-oxide + reduced acceptor
-
?
1.5.99.4 nicotinic acid + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxynicotinic acid + reduced acceptor
-
?
1.5.99.4 nornicotine + acceptor + H2O
-
Pseudarthrobacter oxydans 6-hydroxynornicotine + reduced acceptor
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.99.4 30
-
assay at Pseudarthrobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.99.4 7.6 8.2 sodium diphosphate buffer Pseudarthrobacter oxydans
1.5.99.4 7.9
-
assay at Pseudarthrobacter oxydans
1.5.99.4 8.6 9 Tris chloride or Tris phosphate buffer Pseudarthrobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.99.4 FAD partially protects the enzyme Pseudarthrobacter oxydans
1.5.99.4 FMN metalloflavoprotein, protects enzyme against inhibition by acriflavine Pseudarthrobacter oxydans
1.5.99.4 additional information metalloflavoprotein Pseudarthrobacter oxydans