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Literature summary extracted from

  • Beckmenn, J.D.; Frerman, F.E.
    Electron-transfer flavoprotein-ubiquinone oxidoreductase from pig liver: purification and molecular, redox, and catalytic properties (1985), Biochemistry, 24, 3913-3921.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.5.1 0.00031
-
 hydroquinone electron-transferring flavoprotein
-
 Sus scrofa
1.5.5.1 0.00032
-
 electron-transferring flavoprotein
-
 Sus scrofa
1.5.5.1 0.0077
-
 semiquinone electron-transferring flavoprotein
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.5.1 mitochondrion
-
 Sus scrofa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.5.1 Fe2+/Fe3+ enzyme contains a single 4Fe-1S cluster per subunit and flavin Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.5.1 69000
-
 x * 69000, SDS-PAGE Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.5.5.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.5.1 submitochondrial particles, cholate extract, ammonium sulfate, DEAE-Bio gel, hydroxyapatite Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.5.1 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.5.1 0.093
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.5.5.1 -70°C, 1-2 mg/ml protein, 20% glycerol, several months, no loss in activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.5.1 electron-transferring flavoprotein + hydroquinone electron transferring flavoprotein + ubiquinol
-
 Sus scrofa semiquinone electron transferring flavoprotein + ubiquinone
-
 r
1.5.5.1 reduced electron-transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-pentyl-1,4-benzoquinone
-
 Sus scrofa electron-transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-pentyl-1,4-benzoquinol
-
 r
1.5.5.1 reduced electron-transferring flavoprotein + nitro blue tetrazolium
-
 Sus scrofa electron transferring-flavoprotein + reduced nitro blue tetrazolium
-
 ?
1.5.5.1 semiquinone electron transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-(3-methylbut-2-en)-1,4-benzoquinone disproportion and comproportion with ubiqinone-1 as the terminal oxidant Sus scrofa electron-transferring flavoprotein + hydroquinone electron transferring flavoprotein + 2,3-dimethoxy-5-methyl-6-(3-methylbut-2-en)-1,4-benzoquinol
-
 r
1.5.5.1 semiquinone electron transferring flavoprotein + ubiquinone
-
 Sus scrofa electron-transferring flavoprotein + hydroquinone electron transferring flavoprotein + ubiquinol
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.5.1 ? x * 69000, SDS-PAGE Sus scrofa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.5.1 78
-
 electron-transferring flavoprotein comproportionation of electron-transferring flavoprotein and hydroquinone electron-transferring flavoprotein Sus scrofa
1.5.5.1 200
-
 electron-transferring flavoprotein disproportion of semiquinone electron-transferring flavoprotein Sus scrofa