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Literature summary extracted from

  • Kutzbach, C.; Stokstad, E.L.R.
    10-Formyl tetrahydrofolate:NADP oxidoreductase (1971), Methods Enzymol., 18B, 793-798.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.6 hydroxylamine 0.1 M, strong inhibition Sus scrofa
1.5.1.6 iodoacetamide inhibition reversed by 2-mercaptoethanol Sus scrofa
1.5.1.6 additional information
-
 Rattus norvegicus
1.5.1.6 additional information not inhibited by (+)-10-formyltetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, folate, aminopterin, tetrahydroaminopterin Sus scrofa
1.5.1.6 p-chloromercuribenzoate inhibition reversed by 2-mercaptoethanol Sus scrofa
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus
1.5.1.6 tetrahydrofolate strong product inhibition by both isomers Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.6 0.0035
-
 NADP+
-
 Sus scrofa
1.5.1.6 0.0082
-
 10-formyltetrahydrofolate (-)-isomer Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 320000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Gallus gallus
-
-
-
1.5.1.6 Rattus norvegicus
-
-
-
1.5.1.6 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.6 heart less activity Rattus norvegicus
-
1.5.1.6 kidney less activity Rattus norvegicus
-
1.5.1.6 liver
-
 Gallus gallus
-
1.5.1.6 liver
-
 Rattus norvegicus
-
1.5.1.6 liver
-
 Sus scrofa
-
1.5.1.6 spleen less activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.6 0.98
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.5.1.6 -15°C, presence of 0.01 mM 2-mercaptoethanol, several weeks, stable Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Rattus norvegicus tetrahydrofolate + CO2 + NADPH + H+
-
 ?
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O specific for NADP+ Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ir
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O calculated equilibrium constant: 160000000, specific for (-)-10-formyltetrahydrofolate and NADP+ Sus scrofa tetrahydrofolate + CO2 + NADPH + H+
-
 ir
1.5.1.6 additional information
-
 Rattus norvegicus ?
-
 ?
1.5.1.6 additional information not as substrate: (+)-10-formyltetrahydrofolate Sus scrofa ?
-
 ?
1.5.1.6 additional information not as substrate: 5-formyltetrahydrofolate Sus scrofa ?
-
 ?
1.5.1.6 additional information enzyme catalyzes hydrolytic cleavage of 10-formyltetrahydrofolate to formate and tetrahydrofolate in absence of NADP+ (15-30% of the rate of oxidative reaction) Sus scrofa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.6 30
-
 assay at Rattus norvegicus
1.5.1.6 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.6 7.8
-
-
 Rattus norvegicus
1.5.1.6 7.8
-
 broad pH-optimum centers at pH 7.8 Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.6 6.5 8.5 more than 60% of maximal activity at pH 6.5 and 8.5 Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Rattus norvegicus
1.5.1.6 10-formyltetrahydrofolate 10-formyltetrahydrofolate Sus scrofa
1.5.1.6 NAD+ less than 5% of the activity with NADP+ Sus scrofa
1.5.1.6 NADP+ specific for Rattus norvegicus
1.5.1.6 NADP+ specific for Sus scrofa
1.5.1.6 tetrahydrofolate
-
 Rattus norvegicus
1.5.1.6 tetrahydrofolate
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.6 0.001
-
 tetrahydrofolate (-)-isomer Sus scrofa
1.5.1.6 0.01
-
 tetrahydrofolate (+)-isomer Sus scrofa