Literature summary extracted from

Goenrich, M.; Bursy, J.; Hubner, E.; Linder, D.; Schwartz, A.C.; Vorholt, J.A.
Purification and characterization of the methylene tetrahydromethanopterin dehydrogenase MtdB and the methylene tetrahydrofolate dehydrogenase FolD from Hyphomicrobium zavarzinii ZV580 (2002), Arch. Microbiol., 177, 299-303.

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.5.1.5	0.024	-	5,10-methylenetetrahydrofolate	bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme	Hyphomicrobium zavarzinii
1.5.1.5	0.13	-	NADP+	bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase enzyme	Hyphomicrobium zavarzinii

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.5.1.5	31000	-	x * 31000, SDS-PAGE	Hyphomicrobium zavarzinii

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.5.1.5	5,10-methylenetetrahydrofolate + NADP+	Hyphomicrobium zavarzinii	-	5,10-methenyltetrahydrofolate + NADPH + H+	-	?
1.5.1.5	5,10-methylenetetrahydrofolate + NADP+	Hyphomicrobium zavarzinii ZV580	-	5,10-methenyltetrahydrofolate + NADPH + H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.5.1.5	Hyphomicrobium zavarzinii	-	ZV580	-
1.5.1.5	Hyphomicrobium zavarzinii ZV580	-	ZV580	-
1.5.1.5	no activity in Methylobacterium extorquens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.5.1.5	bifunctional methylenetetrahydrofolate dehydrogenase-cyclohydrolase	Hyphomicrobium zavarzinii

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.5.1.5	additional information	-	-	Hyphomicrobium zavarzinii

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.5.1.5	5,10-methylenetetrahydrofolate + NADP+	-	Hyphomicrobium zavarzinii	5,10-methenyltetrahydrofolate + NADPH + H+	-	?
1.5.1.5	5,10-methylenetetrahydrofolate + NADP+	-	Hyphomicrobium zavarzinii ZV580	5,10-methenyltetrahydrofolate + NADPH + H+	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.5.1.5	?	x * 31000, SDS-PAGE	Hyphomicrobium zavarzinii

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
1.5.1.5	5.5	6.5	-	Hyphomicrobium zavarzinii

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.5.1.5	NADP+	no reduction of NAD+	Hyphomicrobium zavarzinii

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Release 2023.1 (January 2023)