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Literature summary extracted from

  • Dalal, F.R.; Gots, J.S.
    Purification of 5,10-methylenetetrahydrofolate dehydrogenase from Salmonella typhimurium and its inhibition by purine nucleotides (1967), J. Biol. Chem., 242, 3636-3640.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.5.1.5 highly unstable, can be preserved as suspension in ammonium sulfate at 50% saturation Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.5 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.5 GTP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.5 ITP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.5 0.045
-
 5,10-methylenetetrahydrofolate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.5 0.092
-
 NADP+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Salmonella enterica subsp. enterica serovar Typhimurium
-
 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.5 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strain LT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.5
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.5 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.5 160
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
1.5.1.5 5°C, stable for several weeks in ammonium sulfate at 50% saturation Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5,10-methenyltetrahydrofolate + NADPH + H+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.5 7.8
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.5 NADP+
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.5 0.34
-
 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium