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Literature summary extracted from
- The extremely halophilic archaeon Haloferax volcanii has two very different dihydrofolate reductases (2000), Mol. Microbiol., 35, 1493-1505.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.3 | KCl | hDHFR-2, activation is maximal at 0.5 M | Haloferax volcanii |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.5.1.3 | overexpression of hdrB gene in Escherichia coli | Haloferax volcanii |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.5.1.3 | additional information | construction of hdrAgene, hdrB gene and double deletion mutants | Haloferax volcanii |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.5.1.3 | isozyme hDHFR-1, loss of more than 80% activity after exposure to 0.2 M KCl for 24 h at 24°C | Haloferax volcanii |
| 1.5.1.3 | isozyme hDHFR-2 loss of less than 10% activity after exposure to 40 mM KCl for 18 h at 52°C | Haloferax volcanii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.5.1.3 | trimethoprim | hDHFR-1 is much more resistant than hDHFR-2 | Haloferax volcanii | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.5.1.3 | Haloferax volcanii | - |
isozyme hDHFR-1 from gene hdrA and isozyme hDHFR-2 from gene hdrB | - |
| 1.5.1.3 | Haloferax volcanii | - |
extremely halophilic archaebacterium | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.5.1.3 | isozyme hDHFR-2 from hdrB gene overexpressed in Escherichia coli | Haloferax volcanii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Haloferax volcanii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.5.1.3 | hDHFR-1 | - |
Haloferax volcanii |
| 1.5.1.3 | hDHFR-2 | - |
Haloferax volcanii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.5.1.3 | 4 | - |
isozyme hDHFR-1 | Haloferax volcanii |
| 1.5.1.3 | 6 | - |
isozyme hDHFR-2 | Haloferax volcanii |
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