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Literature summary extracted from

  • Rao, K.N.
    Purification and characterization of dihydrofolate reductase from Lactobacillus leichmannii (2000), Indian J. Biochem. Biophys., 37, 121-129.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 CaCl2
-
 Lactobacillus leichmannii
1.5.1.3 KBr
-
 Lactobacillus leichmannii
1.5.1.3 KCl 0.1 M Lactobacillus leichmannii
1.5.1.3 KI
-
 Lactobacillus leichmannii
1.5.1.3 MgCl2
-
 Lactobacillus leichmannii
1.5.1.3 Urea activates at 2 M about 1.5fold Lactobacillus leichmannii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 deoxytubulosine
-
 Lactobacillus leichmannii
1.5.1.3 methotrexate competitive Lactobacillus leichmannii
1.5.1.3 p-chloromercuribenzoate
-
 Lactobacillus leichmannii
1.5.1.3 pegularinine plant alkaloid Lactobacillus leichmannii
1.5.1.3 tylophorinidine plant alkaloid Lactobacillus leichmannii
1.5.1.3 Urea at concentration 3-4 M complete inactivation Lactobacillus leichmannii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0035
-
 NADPH
-
 Lactobacillus leichmannii
1.5.1.3 0.0062
-
 7,8-dihydrofolate
-
 Lactobacillus leichmannii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 CaCl2
-
 Lactobacillus leichmannii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 20000
-
 gel filtration Lactobacillus leichmannii

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Lactobacillus leichmannii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Lactobacillus leichmannii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 200
-
 purified enzyme Lactobacillus leichmannii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Lactobacillus leichmannii 5,6,7,8-tetrahydrofolate + NADP+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.5.1.3 monomer 1 * 20000, SDS-PAGE Lactobacillus leichmannii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 30
-
 assay at Lactobacillus leichmannii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 35
-
 30% loss of activity after 60 min Lactobacillus leichmannii
1.5.1.3 45
-
 90% loss of activity after 30 min Lactobacillus leichmannii
1.5.1.3 50
-
 90% loss of activity after 5 min Lactobacillus leichmannii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7.4
-
-
 Lactobacillus leichmannii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Lactobacillus leichmannii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.000000007
-
 methotrexate
-
 Lactobacillus leichmannii
1.5.1.3 0.005
-
 deoxytubulosine
-
 Lactobacillus leichmannii
1.5.1.3 0.007
-
 tylophorinidine
-
 Lactobacillus leichmannii
1.5.1.3 0.009
-
 pegularinine
-
 Lactobacillus leichmannii