EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | Cl- | - |
Pneumocystis carinii | |
1.5.1.3 | KCl | - |
Rattus norvegicus | |
1.5.1.3 | KCl | recombinant enzyme, concentration 0.1 M | Pneumocystis carinii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | rat-derived gene is overexpressed in Escherichia coli | Pneumocystis carinii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.5.1.3 | crystallization of refolded recombinant enzyme as ternary complex with NADPH and various inhibitors | Pneumocystis carinii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | KCl | recombinant enzyme, concentration above 0.1 M | Pneumocystis carinii | |
1.5.1.3 | methotrexate | recombinant enzyme | Pneumocystis carinii | |
1.5.1.3 | methotrexate | - |
Rattus norvegicus | |
1.5.1.3 | piritrexim | recombinant enzyme | Pneumocystis carinii | |
1.5.1.3 | pyrimethamine | - |
Pneumocystis carinii | |
1.5.1.3 | trimethoprim | - |
Pneumocystis carinii | |
1.5.1.3 | trimetrexate | recombinant enzyme | Pneumocystis carinii | |
1.5.1.3 | Urea | recombinant enzyme, above 4 M | Pneumocystis carinii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | additional information | - |
additional information | buffer and salt conditions significantly alter the Km-values for the substrates | Pneumocystis carinii | |
1.5.1.3 | 0.0023 | - |
7,8-dihydrofolate | recombinant enzyme, pH 7.0, 0.1 M imidazole | Pneumocystis carinii | |
1.5.1.3 | 0.003 | - |
NADPH | recombinant enzyme, pH 7.0, 0.1 M imidazole | Pneumocystis carinii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.5.1.3 | soluble | recombinant enzyme expressed in Escherichia coli | Pneumocystis carinii | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 24000 | - |
recombinant enzyme, gel filtration | Pneumocystis carinii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Pneumocystis carinii | - |
rat-derived | - |
1.5.1.3 | Rattus norvegicus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | recombinant purified from Escherichia coli | Pneumocystis carinii |
EC Number | Renatured (Comment) | Organism |
---|---|---|
1.5.1.3 | refolding after solubilisation from inclusion bodies with 4 M guanidium hydrochloride in 0.5% polyethylene glycol 1450, pH 7.0 | Pneumocystis carinii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.1.3 | lung | source of Pneumocystis carnii | Rattus norvegicus | - |
1.5.1.3 | additional information | derived from rat lung | Pneumocystis carinii | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 42 | - |
recombinant enzyme, partially purified, after refolding | Pneumocystis carinii |
1.5.1.3 | 321 | - |
purified recombinant enzyme | Pneumocystis carinii |
EC Number | Storage Stability | Organism |
---|---|---|
1.5.1.3 | -20°C, 50% glycerol, 30% loss of activity after 9 months | Pneumocystis carinii |
1.5.1.3 | -70°C, 10% glycerol, less than 5% loss of activity after 9 months | Pneumocystis carinii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Rattus norvegicus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Pneumocystis carinii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | folate + NADPH | no activity | Pneumocystis carinii | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.3 | monomer | - |
Rattus norvegicus |
1.5.1.3 | monomer | 1 * 24000, recombinant enzyme, SDS-PAGE | Pneumocystis carinii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 30 | - |
assay at | Pneumocystis carinii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 108 | - |
7,8-dihydrofolate | recombinant enzyme | Pneumocystis carinii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 7.5 | - |
sharp optimum, recombinant enzyme | Pneumocystis carinii |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 6 | 9 | recombinant, refolded enzyme | Pneumocystis carinii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Rattus norvegicus | |
1.5.1.3 | NADPH | - |
Pneumocystis carinii |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.00000000002 | - |
methotrexate | recombinant enzyme | Pneumocystis carinii | |
1.5.1.3 | 0.0007 | - |
pyrimethamine | recombinant enzyme | Pneumocystis carinii |