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Literature summary extracted from

  • Fan, C.C.; Vitols, K.S.; Huennekens, F.M.
    Inhibition of dihydrofolate reductase by methotrexate: a new look at an old problem (1980), Adv. Enzyme Regul., 18, 41-52.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 5,5'-dithiobis(2-nitrobenzoate)
-
 Mus musculus
1.5.1.3 Bromoacetate
-
 Mus musculus
1.5.1.3 diethyldicarbonate
-
 Mus musculus
1.5.1.3 iodoacetamide under high salt condition Mus musculus
1.5.1.3 methotrexate biphasic inhibition at pH 7.0, competitive against 7,8-dihydrofolate; dihydrofolate antagonist drug Mus musculus
1.5.1.3 methylene blue
-
 Mus musculus
1.5.1.3 p-chloromercuribenzoate
-
 Mus musculus
1.5.1.3 Rose bengal
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Mus musculus
-
 multiple forms
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.3 L-1210 cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Mus musculus 5,6,7,8-tetrahydrofolate + NADP+
-
 ir
1.5.1.3 folate + NADPH slow rate Mus musculus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Mus musculus