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Literature summary extracted from

  • Bachmann, B.; Follmann, H.
    Deoxyribonucleotide biosynthesis in green algae: characterization of thymidylate synthase-dihydrofolate reductase in Scenedesmus obliquus (1987), Arch. Biochem. Biophys., 256, 244-252.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.45 -80°C, storage in 30% glycerol stabilizes enzyme Tetradesmus obliquus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 folate
-
 Tetradesmus obliquus
1.5.1.3 methotrexate
-
 Tetradesmus obliquus
1.5.1.3 additional information thymidylate synthase activity inhibitors Tetradesmus obliquus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Daucus carota
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Leishmania major
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Mus musculus
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Saccharomyces cerevisiae
2.1.1.45 5-fluoro-2'-deoxyuridylic acid
-
 Tetradesmus obliquus
2.1.1.45 Arabinouridylate
-
 Tetradesmus obliquus
2.1.1.45 Hydroxyurea supresses thymidilate synthase in growing algae, but no effect on purified enzyme Tetradesmus obliquus
2.1.1.45 methotrexate
-
 Saccharomyces cerevisiae
2.1.1.45 methotrexate
-
 Tetradesmus obliquus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 50000
-
 2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE Tetradesmus obliquus
1.5.1.3 100000
-
 thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, sucrose density gradient centrifugation Tetradesmus obliquus
2.1.1.45 50000
-
 2 * 50000, SDS-PAGE Tetradesmus obliquus
2.1.1.45 56000
-
 2 * 56000 Leishmania major
2.1.1.45 100000
-
 sedimentation in sucrose density gradients Tetradesmus obliquus
2.1.1.45 110000
-
-
 Leishmania major

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Chlamydomonas reinhardtii
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Tetradesmus obliquus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Mus musculus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Saccharomyces cerevisiae
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Bos taurus
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Nicotiana tabacum
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Daucus carota
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Leishmania major
-
 dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP Abies sp.
-
 dihydrofolate + dTMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Tetradesmus obliquus
-
 green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
-
2.1.1.45 Abies sp.
-
 pine
-
2.1.1.45 Bos taurus
-
 calf
-
2.1.1.45 Chlamydomonas reinhardtii
-
-
-
2.1.1.45 Daucus carota
-
 carrot, wild and culture form
-
2.1.1.45 Leishmania major
-
-
-
2.1.1.45 Mus musculus
-
-
-
2.1.1.45 Nicotiana tabacum
-
 tobacco
-
2.1.1.45 Saccharomyces cerevisiae
-
 bakers' yeast
-
2.1.1.45 Tetradesmus obliquus
-
 unicellular green algae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other Tetradesmus obliquus
2.1.1.45
-
 Tetradesmus obliquus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.45 thymus
-
 Mus musculus
-
2.1.1.45 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 15.8
-
-
 Tetradesmus obliquus
2.1.1.45 0.0257
-
-
 Tetradesmus obliquus

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 5°C, enzyme after affinity chromatography, half-life 2 days Tetradesmus obliquus
2.1.1.45 -80°C, storage in 30% glycerol stabilizes enzyme Tetradesmus obliquus
2.1.1.45 5°C half-life in buffered solution only 2 days Tetradesmus obliquus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Tetradesmus obliquus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Chlamydomonas reinhardtii dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Chlamydomonas reinhardtii dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tetradesmus obliquus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Tetradesmus obliquus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Mus musculus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Saccharomyces cerevisiae dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Bos taurus dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Nicotiana tabacum dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Nicotiana tabacum dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Daucus carota dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Daucus carota dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Leishmania major dihydrofolate + dTMP
-
 r
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Abies sp. dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Abies sp. dihydrofolate + dTMP
-
 r
2.1.1.45 additional information bifunctional enzyme thymidilate synthase-dihydrofolate reductase, not possible to separate the 2 activities Tetradesmus obliquus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 dimer 2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE Tetradesmus obliquus
2.1.1.45 dimer
-
 Mus musculus
2.1.1.45 dimer
-
 Saccharomyces cerevisiae
2.1.1.45 dimer
-
 Bos taurus
2.1.1.45 dimer 2 * 50000, SDS-PAGE Tetradesmus obliquus
2.1.1.45 dimer 2 * 56000 Leishmania major

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 25 30
-
 Tetradesmus obliquus
1.5.1.3 25
-
 assay at Tetradesmus obliquus
2.1.1.45 25 30
-
 Tetradesmus obliquus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7.5
-
 thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other Tetradesmus obliquus
2.1.1.45 7.5
-
-
 Tetradesmus obliquus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 6 9 activity negligible below and above Tetradesmus obliquus
2.1.1.45 6 9 activity is negligible below pH 6.0 and above pH 9.0 Tetradesmus obliquus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Tetradesmus obliquus