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Literature summary extracted from

  • Al-Rubeai, M.; Dale, J.W.
    Purification and characterization of dihydrofolate reductase from Mycobacterium phlei (1986), Biochem. J., 235, 301-303.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Mycolicibacterium phlei

General Stability

EC Number General Stability Organism
1.5.1.3 bovine serum albumin stabilizes purified enzyme Mycolicibacterium phlei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
Mycolicibacterium phlei
1.5.1.3 trimethoprim
-
Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0086
-
7,8-dihydrofolate
-
Mycolicibacterium phlei
1.5.1.3 0.035
-
NADPH
-
Mycolicibacterium phlei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 15000
-
SDS-PAGE Mycolicibacterium phlei

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Mycolicibacterium phlei
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 additional information
-
-
Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
Mycolicibacterium phlei 5,6,7,8-tetrahydrofolate + NADP+
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.3 30
-
assay at Mycolicibacterium phlei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.3 45
-
3.5 h, no loss of activity in presence of bovine serum albumin Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7
-
-
Mycolicibacterium phlei

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
Mycolicibacterium phlei