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Literature summary extracted from

  • Joyner, S.S.; Fling, M.E.; Stone, D.; Baccanari, D.P.
    Characterization of an R-plasmid dihydrofolate reductase with a monomeric structure (1984), J. Biol. Chem., 259, 5851-5856.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 trimethoprim
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 additional information
-
 additional information
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 0.0004
-
 7,8-dihydrofolate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 additional information
-
 overview Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 16700
-
 PAGE under denaturating and nondenaturating conditions Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 16900
-
 1 * 16900, R-plasmid enzyme, PAGE under denaturating and nondenaturating conditions Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
 phage type 179, R-plasmid enzyme, isozyme type III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 phage type 179, R-plasmid enzyme, isozyme type III Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
1.5.1.3 4°C, pH 7, 20% glycerol, 1 mM EDTA, 1 mM dithiothreitol, at least 90% of activity retained, 2-3 weeks Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 monomer 1 * 16900, R-plasmid enzyme, PAGE under denaturating and nondenaturating conditions Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.2
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 7.8
-
 R-plasmid encoded enzyme, 2 optima: pH 4.2 and pH 7.8 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 additional information
-
 additional information overview Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 0.000000009
-
 methotrexate isozyme type III, R-plasmid encoded Salmonella enterica subsp. enterica serovar Typhimurium
1.5.1.3 0.000019
-
 trimethoprim isozyme type III, R-plasmid encoded Salmonella enterica subsp. enterica serovar Typhimurium