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Literature summary extracted from

  • Nixon, P.F.; Blakley, R.J.
    Dihydrofolate reductase of Streptococcus faecium. II. Purification and some properties of two dihydrofolate reductases from the amethopterin-resistant mutant Streptococcus faecium var. durans strain A (1968), J. Biol. Chem., 243, 4722-4731.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.3 NH4+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 Cs+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 K+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 Li+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 methopterin
-
 Enterococcus faecium
1.5.1.3 Na+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 NH4+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 p-mercuribenzoate mutant enzyme: no effect, wild type: inhibition Enterococcus faecium
1.5.1.3 phenylmercuric acetate mutant enzyme: no effect, wild type enzyme: inhibition Enterococcus faecium
1.5.1.3 Rb+ activation of mutant type enzyme at 0.2 mM, decrease of wild-type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 Urea inactivation Enterococcus faecium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.3 Cs+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 K+ activation of mutant type enzyme activity at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 Li+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 Na+ activation of mutant type enzyme 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium
1.5.1.3 Rb+ activation of mutant type enzyme at 0.2 mM, decrease of wild type enzyme activity above 0.1 mM Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 20000
-
 velocity sedimentation, mutant type Enterococcus faecium
1.5.1.3 28000
-
 wild type, gel filtration and velocity sedimentation Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Enterococcus faecium
-
 2 forms termed mutant type and wild-type enzyme in a single organism
-
1.5.1.3 Enterococcus faecium
-
 var. durans
-
1.5.1.3 Enterococcus faecium
-
 amethopterin-resistant strain
-
1.5.1.3 Enterococcus faecium A
-
 var. durans
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3 2 forms: mutant-type and wild type enzyme in a single organism Enterococcus faecium
1.5.1.3 amethopterin-resistant strain Enterococcus faecium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.3 285
-
-
 Enterococcus faecium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Enterococcus faecium A 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 folate + NADPH
-
 Enterococcus faecium A 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 0.125
-
 folate mutant type Enterococcus faecium
1.5.1.3 15
-
 7,8-dihydrofolate mutant type Enterococcus faecium
1.5.1.3 133
-
 7,8-dihydrofolate wild-type Enterococcus faecium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.7
-
 folate, mutant type enzyme Enterococcus faecium
1.5.1.3 5.8
-
 wild type, mutant type Enterococcus faecium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.3 additional information
-
-
 Enterococcus faecium

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Enterococcus faecium