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Literature summary extracted from

  • Roth, B.; Bliss, E.; Beddell, C.R.
    Inhibitors of dihydrofolate reductase (1983), Top. Mol. Struct. Biol., 3, 363-393.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Gallus gallus
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Escherichia coli
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Homo sapiens
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Sus scrofa
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Bos taurus
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Lacticaseibacillus casei
1.5.1.3 medicine enzyme is a target of several anti-folate inhibitory drugs to combat bacteria, protozoa and cancer Enterococcus faecium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.3 crystal structure, complex with 2,4-diamino-5,6-dihydro-6,6-dimethyl-5-(4'-methoxyphenyl)-s-triazine Gallus gallus
1.5.1.3 structure of ternary complex with NADPH and methotrexate Escherichia coli
1.5.1.3 structure of ternary complex with NADPH and methotrexate Lacticaseibacillus casei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 2,4-diamino-5,6-dihydro-6,6-dimethyl-5-(4'-methoxyphenyl)-s-triazine
-
 Gallus gallus
1.5.1.3 methotrexate
-
 Bos taurus
1.5.1.3 methotrexate dihydrofolate antagonist drug Escherichia coli
1.5.1.3 methotrexate
-
 Gallus gallus
1.5.1.3 methotrexate dihydrofolate antagonist drug Homo sapiens
1.5.1.3 methotrexate
-
 Lacticaseibacillus casei
1.5.1.3 methotrexate
-
 Sus scrofa
1.5.1.3 additional information overview Bos taurus
1.5.1.3 additional information overview Enterococcus faecium
1.5.1.3 additional information overview Escherichia coli
1.5.1.3 additional information overview Gallus gallus
1.5.1.3 additional information overview Lacticaseibacillus casei
1.5.1.3 additional information overview Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Bos taurus
-
 overview
-
1.5.1.3 Enterococcus faecium
-
 overview
-
1.5.1.3 Escherichia coli
-
-
-
1.5.1.3 Gallus gallus
-
 overview
-
1.5.1.3 Homo sapiens
-
 methotrexate-resistant WIL-2 lymphoblastoid cells
-
1.5.1.3 Lacticaseibacillus casei
-
-
-
1.5.1.3 Sus scrofa
-
 overview
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Gallus gallus
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Escherichia coli
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Homo sapiens
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Sus scrofa
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Bos taurus
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Lacticaseibacillus casei
a
1.5.1.3 5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH + H+ inhibitor binding mechanism Enterococcus faecium
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Gallus gallus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Homo sapiens 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Sus scrofa 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Bos taurus 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Lacticaseibacillus casei 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 7,8-dihydrofolate + NADPH
-
 Enterococcus faecium 5,6,7,8-tetrahydrofolate + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Gallus gallus
1.5.1.3 NADPH
-
 Escherichia coli
1.5.1.3 NADPH
-
 Homo sapiens
1.5.1.3 NADPH
-
 Sus scrofa
1.5.1.3 NADPH
-
 Bos taurus
1.5.1.3 NADPH
-
 Lacticaseibacillus casei
1.5.1.3 NADPH
-
 Enterococcus faecium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.0000037
-
 2,4-diamino-5,6-dihydro-6,6-dimethyl-5-(4'-methoxyphenyl)-s-triazine
-
 Gallus gallus