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Literature summary extracted from

  • Jablonski, E.; DeLuca, M.
    Purification and properties of the NADH and NADPH specific FMN oxidoreductases from Beneckea harveyi (1977), Biochemistry, 16, 2932-2936.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.38 1,10-phenanthroline 0.005 M, 14% inhibition Vibrio harveyi
1.5.1.38 AMP 0.01 M, 28% inhibition Vibrio harveyi
1.5.1.38 dicoumarol 0.000325 M, 15% inhibition Vibrio harveyi
1.5.1.38 KCN 0.01 M, 10% inhibition Vibrio harveyi
1.5.1.38 N-ethylmaleimide 0.001 M, 26% inhibition Vibrio harveyi
1.5.1.38 p-hydroxymercuribenzoate 0.001 M, 80% inhibition Vibrio harveyi
1.5.1.38 rotenone 0.0004 M, 30% inhibition Vibrio harveyi
1.5.1.42 1,10-phenanthroline 0.005 M, 52% inhibition Vibrio harveyi
1.5.1.42 5,5'-dithiobis(2-nitrobenzoic acid) 0.001 M, 38% inhibition Vibrio harveyi
1.5.1.42 AMP 0.01 M, 36% inhibition Vibrio harveyi
1.5.1.42 additional information no inhibition by: 0.000325 M dicoumarol, 10 mM KCN, 1% Triton X-100 Vibrio harveyi
1.5.1.42 N-ethylmaleimide 0.001 M, 38% inhibition Vibrio harveyi
1.5.1.42 p-hydroxymercuribenzoate 0.001 M, 49% inhibition Vibrio harveyi
1.5.1.42 rotenone 0.0004 M, 20% inhibition Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.38 0.014
-
 FMN pH 7.0, 23°C, spectrometric assay Vibrio harveyi
1.5.1.38 0.018
-
 FMN pH 5.6, 23°C, spectrometric assay Vibrio harveyi
1.5.1.38 0.02
-
 NADPH pH 5.6, 23°C, spectrometric assay Vibrio harveyi
1.5.1.38 0.04
-
 NADPH pH 7.0, 23°C Vibrio harveyi
1.5.1.38 0.04
-
 NADPH pH 7.0, 23°C, spectrometric assay Vibrio harveyi
1.5.1.42 0.001
-
 FMN pH 5.6, 23°C, spectrometric assay Vibrio harveyi
1.5.1.42 0.001
-
 FMN pH 8.5, 23°C, spectrometric assay Vibrio harveyi
1.5.1.42 0.012
-
 NADH pH 8.5, 23°C, spectrometric assay Vibrio harveyi
1.5.1.42 0.047
-
 NADH pH 5.6, 23°C, spectrometric assay Vibrio harveyi
1.5.1.42 0.0475
-
 NADH pH 7.0, 23°C Vibrio harveyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.38 40000
-
 gel filtration Vibrio harveyi
1.5.1.42 30000
-
 gel filtration Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.38 FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+ Vibrio harveyi No. 392
-
 FMNH2 + NADP+
-
 ?
1.5.1.42 FMN + NADPH + H+ Vibrio harveyi
-
 FMNH2 + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.38 Vibrio harveyi
-
-
-
1.5.1.38 Vibrio harveyi No. 392
-
-
-
1.5.1.42 Vibrio harveyi
-
-
-
1.5.1.42 Vibrio harveyi No. 392
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.38
-
 Vibrio harveyi
1.5.1.42
-
 Vibrio harveyi

Reaction

EC Number Reaction Comment Organism Reaction ID
1.5.1.38 FMNH2 + NADP+ = FMN + NADPH + H+ the enzyme exhibits double displacement or ping-pong kinetics Vibrio harveyi
a
1.5.1.42 FMNH2 + NAD+ = FMN + NADH + H+ the enzyme exhibits single displacement kinetics Vibrio harveyi
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.38 51
-
 pH 7.0, 23°C Vibrio harveyi
1.5.1.42 31
-
 pH 7.0, 23°C Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.38 FAD + NADPH + H+
-
 Vibrio harveyi FADH2 + NADP+
-
 ?
1.5.1.38 FAD + NADPH + H+
-
 Vibrio harveyi No. 392 FADH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+ the NADPH specific FMN reductase does not utilize NADH Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+
-
 Vibrio harveyi No. 392 FMNH2 + NADP+
-
 ?
1.5.1.38 FMN + NADPH + H+ the NADPH specific FMN reductase does not utilize NADH Vibrio harveyi No. 392 FMNH2 + NADP+
-
 ?
1.5.1.38 additional information the enzyme also exhibits appreciable activity with some artificial acceptors: menadione, 2,6-dichlorophenolindophenol, KFeCN6 or 5,5'-dithiobis(2-nitrobenzoic acid). Low activity with methylene blue as acceptor Vibrio harveyi ?
-
 ?
1.5.1.38 additional information the enzyme also exhibits appreciable activity with some artificial acceptors: menadione, 2,6-dichlorophenolindophenol, KFeCN6 or 5,5'-dithiobis(2-nitrobenzoic acid). Low activity with methylene blue as acceptor Vibrio harveyi No. 392 ?
-
 ?
1.5.1.42 FAD + NADH + H+ activity is 75% of that with FMN Vibrio harveyi FADH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+
-
 Vibrio harveyi FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+ the NADH specific FMN reductase does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH Vibrio harveyi FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADH + H+
-
 Vibrio harveyi No. 392 FMNH2 + NAD+
-
 ?
1.5.1.42 FMN + NADPH + H+
-
 Vibrio harveyi FMNH2 + NADP+
-
 ?
1.5.1.42 FMN + NADPH + H+ the NADH specific FMN reductase does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH Vibrio harveyi FMNH2 + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.38 NADPH specific FMN reductase
-
 Vibrio harveyi
1.5.1.38 NADPH:FMN oxidoreductase
-
 Vibrio harveyi
1.5.1.42 NADH specific FMN reductase
-
 Vibrio harveyi
1.5.1.42 NADH:FMN oxidoreductase
-
 Vibrio harveyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.38 23
-
 assay at Vibrio harveyi
1.5.1.42 23
-
 assay at Vibrio harveyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.38 34
-
 NADPH pH 7.0, 23°C Vibrio harveyi
1.5.1.42 15.5
-
 NADPH pH 7.0, 23°C Vibrio harveyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.38 5.5
-
-
 Vibrio harveyi
1.5.1.38 7
-
 assay at Vibrio harveyi
1.5.1.42 5.5
-
-
 Vibrio harveyi
1.5.1.42 7
-
 assay at Vibrio harveyi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.38 5 10 the enzyme exhibits a maximum activity at pH 5.5 which drops to a broad shoulder from pH 6.5 to pH 8.5 with an activity 75% that of maximum at pH 7.0. About 60% of maximal activity at pH 5.0, about 50% of maximal activity at pH 10.0 Vibrio harveyi
1.5.1.42 5 8.6 the pH curve is bimodal, with maximal activity exhibited at pH 8.6, a minimum at pH 6.0. and a second maximum at pH 5.0 Vibrio harveyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.38 NADPH the NADPH specific FMN reductase does not utilize NADH Vibrio harveyi
1.5.1.42 NADPH the NADH specific FMN reductase does does dehydrogenate NADPH with a maximal velocity one-tenth of that for NADH Vibrio harveyi