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Literature summary extracted from

  • Basch, J.J.; Wickham, E.D.; Farrell, H.M., Jr.
    Pyrroline-5-carboxylate reductase in lactating bovine mammary glands (1996), J. Dairy Sci., 79, 1361-1368.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.2 putrescine 11% activation at 5 mM Bos taurus
1.5.1.2 spermidine 10% activation at 1.2 mM Bos taurus
1.5.1.2 spermine 13% activation at 1.2 mM Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 additional information
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.2 additional information
-
 additional information
-
 Bos taurus
1.5.1.2 0.37
-
 1-pyrroline-5-carboxylate with NADPH Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.2 cytosol
-
 Bos taurus 5829
-
1.5.1.2 mitochondrion
-
 Bos taurus 5739
-

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 mammary gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
1.5.1.2 -80°C, 1 year Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.2 1-pyrroline-5-carboxylate + NADPH + H+
-
 Bos taurus L-proline + NADP+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2 37
-
 4 h stable Bos taurus
1.5.1.2 100
-
 heating for 4 min completely destroys the enzyme Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 6.05
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.2 6 7
-
 Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.2 NADH
-
 Bos taurus
1.5.1.2 NADPH
-
 Bos taurus