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Literature summary extracted from

  • Rena, A.B.; Splittstoesser, W.E.
    Proline dehydrogenase and pyrroline-5-carboxylate reductase from pumpkin cotylenons (1975), Phytochemistry, 14, 657-661.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.2 ADP
-
 Cucurbita moschata
1.5.1.2 Ag+ 44% inactivation at 0.7 mM Cucurbita moschata
1.5.1.2 ATP
-
 Cucurbita moschata
1.5.1.2 Cu2+ not Cucurbita moschata
1.5.1.2 cysteine 77% inactivation at 10 mM Cucurbita moschata
1.5.1.2 Hg2+
-
 Cucurbita moschata
1.5.1.2 iodoacetate 10% inactivation at 1 mM Cucurbita moschata
1.5.1.2 NaHSO3 88% inactivation at 1 mM Cucurbita moschata
1.5.1.2 NH2OH 7% inactivation at 1 mM Cucurbita moschata
1.5.1.2 p-chloromercuribenzoate not Cucurbita moschata
1.5.1.2 proline
-
 Cucurbita moschata
1.5.1.2 Sodium bisulfite
-
 Cucurbita moschata
1.5.1.2 Zn2+ 14% inactivation at 1 mM Cucurbita moschata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.2 100000
-
 gel filtration Cucurbita moschata

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.2 Cucurbita moschata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.2 copurification with proline oxidase Cucurbita moschata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.2 cotyledon
-
 Cucurbita moschata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.2 2070
-
-
 Cucurbita moschata

Storage Stability

EC Number Storage Stability Organism
1.5.1.2 3°C, 0.1 M sodium phosphate buffer pH 7.6, 10% (NH4)2SO4, 7 days Cucurbita moschata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.2 65
-
 15 min stable Cucurbita moschata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.2 6.5
-
 pyrroline-5-carboxylate reductase activity Cucurbita moschata
1.5.1.2 9.8 10.4 proline dehydrogenase activity Cucurbita moschata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.2 4 8 pyrroline-5-carboxylate reductase activity Cucurbita moschata
1.5.1.2 9.2 10.5 proline dehydrogenase activity Cucurbita moschata