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Literature summary extracted from

  • Mejia, N.; Rios-Orlandi, E.M.; Mackenzie, R.E.
    NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase from ascites tumor cells. Purification and properties (1986), J. Biol. Chem., 261, 9509-9513.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.15 arsenate 0.8 mM 104% activity Mus musculus
1.5.1.15 phosphate
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.15 0.019
-
L-5,10-methylenetetrahydrofolate
-
Mus musculus
1.5.1.15 0.111
-
NAD+
-
Mus musculus
1.5.1.15 0.15
-
5,10-methenyltetrahydrofolate
-
Mus musculus
1.5.1.15 0.17
-
phosphate
-
Mus musculus
1.5.1.15 0.25
-
Mg2+
-
Mus musculus
1.5.1.15 0.793
-
arsenate
-
Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.15 Ca2+ can substitute for Mg2+ Mus musculus
1.5.1.15 Mg2+
-
Mus musculus
1.5.1.15 Mn2+
-
Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.15 34000
-
SDS-PAGE Mus musculus
1.5.1.15 68000
-
cross-linking with bis(sulfosuccinimidyl)suberate Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Mus musculus
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Clostridium formicaceticum
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH Acetobacterium woodii
-
L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Mus musculus
-
5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Clostridium formicaceticum
-
5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+ Acetobacterium woodii
-
5,10-methenyl-tetrahydrofolate + NADH
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.15 Acetobacterium woodii
-
-
-
1.5.1.15 Clostridium formicaceticum
-
-
-
1.5.1.15 Mus musculus
-
mouse, Ehrlich ascites tumor cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.15 from ascites tumor cells Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.15 38
-
-
Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Mus musculus L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Clostridium formicaceticum L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methenyl-tetrahydrofolate + NADH
-
Acetobacterium woodii L-5,10-methylenetetrahydrofolate + NAD+
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Mus musculus 5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Clostridium formicaceticum 5,10-methenyl-tetrahydrofolate + NADH
-
r
1.5.1.15 L-5,10-methylenetetrahydrofolate + NAD+
-
Acetobacterium woodii 5,10-methenyl-tetrahydrofolate + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.5.1.15 dimer
-
Clostridium formicaceticum
1.5.1.15 dimer
-
Acetobacterium woodii
1.5.1.15 dimer 2 * 34000, native bifunctional enzyme, associated with cyclohydrolase, SDS-PAGE Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.15 NAD+
-
Mus musculus
1.5.1.15 NAD+
-
Clostridium formicaceticum
1.5.1.15 NAD+
-
Acetobacterium woodii