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Literature summary extracted from

  • Menzel, R.; Roth, J.
    Purification of the putA gene product. A bifunctional membrane-bound protein from Salmonella typhimurium responsible for the two-step oxidation of proline to glutamate (1981), J. Biol. Chem., 256, 9755-9761.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.88 additional information
-
 additional information
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.88 additional information
-
 putA gene product, different forms by native PAGE with 2 activities: proline oxidase and 1-pyrroline-5-carboxylate dehydrogenase activity Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.88 132000
-
 2 * 132000, detergent solubilized, disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.88 132000
-
 1 * 132000, membrane bound in vivo, disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.88 Salmonella enterica subsp. enterica serovar Typhimurium
-
 putA gene product: enzyme with 2 activities: proline oxidase and 1-pyrroline-5-carboxylate dehydrogenase, which require different reaction conditions
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.88 copurification with proline oxidase Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.88 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.88 104
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
1.2.1.88 0°C, 70 mM Tris-HCl buffer, pH 8.2, 30% v/v glycerol, several weeks Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.88 1-pyrroline-5-carboxylate + NAD+ + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-glutamate + NADH
-
 ir
1.2.1.88 additional information enzyme with 2 activities: proline oxidase and 1-pyrroline-5-carboxylate dehydrogenase, which require different reaction conditions Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.88 dimer 2 * 132000, detergent solubilized, disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium
1.2.1.88 monomer 1 * 132000, membrane bound in vivo, disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium