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Literature summary extracted from

  • Blumer, C.; Haas, D.
    Iron regulation of the hcnABC genes encoding hydrogen cyanide synthase depends on the anaerobic regulator ANR rather than on the global activator GacA in Pseudomonas fluorescens CHA0 (2000), Microbiology, 146, 2417-2424.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.99.5 additional information ANR, anaerobic regulator required for maximal expression of the HCN biosynthetic genes Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.99.5 hcnABC gene cluster encoding hydrogen cyanide synthase, heterologously expressed in Escherichia coli Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.99.5 O2
-
 Pseudomonas aeruginosa
1.4.99.5 O2 very sensitive to molecular oxygen, becomes rapidly inactivated in presence Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.99.5 Fe2+ iron regulation of the hcnABC genes encoding hydrogen cyanide synthase, iron limitation has negative effect on hcn expression Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.99.5 glycine + O2 Pseudomonas fluorescens
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas aeruginosa
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Chromobacterium violaceum
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas aeruginosa PAO1A4
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas fluorescens CHA0
-
 HCN + CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.5 Chromobacterium violaceum
-
-
-
1.4.99.5 Pseudomonas aeruginosa
-
-
-
1.4.99.5 Pseudomonas aeruginosa
-
 wild-type PAO1
-
1.4.99.5 Pseudomonas aeruginosa PAO1A4
-
-
-
1.4.99.5 Pseudomonas fluorescens
-
-
-
1.4.99.5 Pseudomonas fluorescens CHA0
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Chromobacterium violaceum HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa PAO1A4 HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens CHA0 HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Chromobacterium violaceum HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa PAO1A4 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens CHA0 HCN + CO2 + 2 H2O
-
 ?