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Literature summary extracted from

  • Laville, J.; Blumer, C.; von Schroetter, C.; Gaia, V.; Defago, G.; Keel, C.; Haas, D.
    Characterization of the HCNABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0 (1998), J. Bacteriol., 180, 3187-3196.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.99.5 FAD stimulates the reaction Pseudomonas sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.99.5 hcnABC gene cluster encoding hydrogen cyanide synthase, heterologously expressed in Escherichia coli Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.99.5 O2
-
 Pseudomonas aeruginosa
1.4.99.5 O2 very sensitive to molecular oxygen, becomes rapidly inactivated in presence Pseudomonas fluorescens
1.4.99.5 O2 very sensitive to molecular oxygen, becomes rapidly inactivated in presence Pseudomonas sp.
1.4.99.5 pyrrolnitrin strongly inhibits cyanide formation in vitro Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.99.5 membrane membrane-bound flavoenzyme Pseudomonas fluorescens 16020
-
1.4.99.5 membrane membrane-bound flavoenzyme Pseudomonas aeruginosa 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.99.5 glycine + O2 Pseudomonas fluorescens
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas aeruginosa
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas sp.
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Chromobacterium violaceum
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas aeruginosa ADD1976
-
 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2 Pseudomonas fluorescens CHA0
-
 HCN + CO2 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.99.5 Chromobacterium violaceum
-
-
-
1.4.99.5 Pseudomonas aeruginosa
-
-
-
1.4.99.5 Pseudomonas aeruginosa
-
 wild-type PAO1
-
1.4.99.5 Pseudomonas aeruginosa ADD1976
-
-
-
1.4.99.5 Pseudomonas fluorescens
-
-
-
1.4.99.5 Pseudomonas fluorescens
-
 wild-type P3
-
1.4.99.5 Pseudomonas fluorescens
-
 mutant CHA5, CHA21, CHA77
-
1.4.99.5 Pseudomonas fluorescens CHA0
-
-
-
1.4.99.5 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.99.5 partially Pseudomonas aeruginosa
1.4.99.5 partially Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas sp. HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Chromobacterium violaceum HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa ADD1976 HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens CHA0 HCN + CO2 + H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas sp. HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Chromobacterium violaceum HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas aeruginosa ADD1976 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + O2
-
 Pseudomonas fluorescens CHA0 HCN + CO2 + 2 H2O
-
 ?
1.4.99.5 glycine + phenazine methosulfate
-
 Pseudomonas aeruginosa HCN + CO2 + ?
-
 ?
1.4.99.5 glycine + phenazine methosulfate
-
 Pseudomonas sp. HCN + CO2 + ?
-
 ?
1.4.99.5 glycine + phenazine methosulfate
-
 Pseudomonas aeruginosa ADD1976 HCN + CO2 + ?
-
 ?