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Literature summary extracted from

  • Motokawa, Y.; Kikuchi, G.
    Glycine metabolism by rat liver mitochondria. Reconstruction of the reversible glycine cleavage system with partially purified protein components (1974), Arch. Biochem. Biophys., 164, 624-633.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.4.2 N-ethylmaleimide
-
Rattus norvegicus
2.1.2.10 iodoacetamide 5 mM, 70% inhibition Rattus norvegicus
2.1.2.10 N-ethylmaleimide 0.1 mM, 70% inhibition Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.4.2 mitochondrion
-
Rattus norvegicus 5739
-
2.1.2.10 mitochondrial inner membrane
-
Rattus norvegicus 5743
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.10 33000
-
gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.4.2 glycine + lipoylprotein Rattus norvegicus lipoyl protein: H-protein, lipoamide can also act as acceptor S-aminomethyldihydrolipoylprotein + CO2
-
?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate Rattus norvegicus glycine metabolism dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.4.2 Rattus norvegicus
-
-
-
2.1.2.10 Rattus norvegicus
-
male Wistar rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.4.2
-
Rattus norvegicus
2.1.2.10
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.4.2 liver
-
Rattus norvegicus
-
2.1.2.10 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.4.2 0.053
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.4.4.2 0-20°C, 24 h, P-protein in purified P,L-protein fraction, 50-60% loss of activity Rattus norvegicus
2.1.2.10 -20°C, 24 h, 60% loss of activity Rattus norvegicus
2.1.2.10 0-4°C, 1 week, stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Rattus norvegicus S-aminomethyldihydrolipoylprotein + CO2
-
?
1.4.4.2 additional information the enzyme is a component of the reversible glycine cleavage system, previously known as glycine synthase Rattus norvegicus ?
-
?
2.1.2.10 additional information enzyme is a component of the reversible glycine cleavage system which is composed of 4 protein components named as P-, H-, L- and T-protein Rattus norvegicus ?
-
?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates Rattus norvegicus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
r
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate T-protein participates in the formation of the one carbon unit and ammonia or the reverse reaction Rattus norvegicus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
r
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate glycine metabolism Rattus norvegicus dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.10 37
-
assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.4.2 70
-
1 min, complete loss of activity Rattus norvegicus
2.1.2.10 50
-
1 min, 20% loss of activity Rattus norvegicus
2.1.2.10 70
-
1 min, complete loss of activity Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.4.2 pyridoxal 5'-phosphate
-
Rattus norvegicus
2.1.2.10 tetrahydrofolate
-
Rattus norvegicus