EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.4.2 | N-ethylmaleimide | - |
Rattus norvegicus | |
2.1.2.10 | iodoacetamide | 5 mM, 70% inhibition | Rattus norvegicus | |
2.1.2.10 | N-ethylmaleimide | 0.1 mM, 70% inhibition | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.4.4.2 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
2.1.2.10 | mitochondrial inner membrane | - |
Rattus norvegicus | 5743 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.2.10 | 33000 | - |
gel filtration | Rattus norvegicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.4.2 | glycine + lipoylprotein | Rattus norvegicus | lipoyl protein: H-protein, lipoamide can also act as acceptor | S-aminomethyldihydrolipoylprotein + CO2 | - |
? | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | Rattus norvegicus | glycine metabolism | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.4.2 | Rattus norvegicus | - |
- |
- |
2.1.2.10 | Rattus norvegicus | - |
male Wistar rats | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.4.2 | - |
Rattus norvegicus |
2.1.2.10 | - |
Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.4.2 | liver | - |
Rattus norvegicus | - |
2.1.2.10 | liver | - |
Rattus norvegicus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.4.4.2 | 0.053 | - |
- |
Rattus norvegicus |
EC Number | Storage Stability | Organism |
---|---|---|
1.4.4.2 | 0-20°C, 24 h, P-protein in purified P,L-protein fraction, 50-60% loss of activity | Rattus norvegicus |
2.1.2.10 | -20°C, 24 h, 60% loss of activity | Rattus norvegicus |
2.1.2.10 | 0-4°C, 1 week, stable | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.4.2 | glycine + lipoylprotein | lipoyl protein: H-protein, lipoamide can also act as acceptor | Rattus norvegicus | S-aminomethyldihydrolipoylprotein + CO2 | - |
? | |
1.4.4.2 | additional information | the enzyme is a component of the reversible glycine cleavage system, previously known as glycine synthase | Rattus norvegicus | ? | - |
? | |
2.1.2.10 | additional information | enzyme is a component of the reversible glycine cleavage system which is composed of 4 protein components named as P-, H-, L- and T-protein | Rattus norvegicus | ? | - |
? | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | decarboxylated glycine moiety attached to H-protein + tetrahydrofolate as substrates | Rattus norvegicus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | T-protein participates in the formation of the one carbon unit and ammonia or the reverse reaction | Rattus norvegicus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
r | |
2.1.2.10 | S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate | glycine metabolism | Rattus norvegicus | dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3 | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.2.10 | 37 | - |
assay at | Rattus norvegicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.4.2 | 70 | - |
1 min, complete loss of activity | Rattus norvegicus |
2.1.2.10 | 50 | - |
1 min, 20% loss of activity | Rattus norvegicus |
2.1.2.10 | 70 | - |
1 min, complete loss of activity | Rattus norvegicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.4.2 | pyridoxal 5'-phosphate | - |
Rattus norvegicus | |
2.1.2.10 | tetrahydrofolate | - |
Rattus norvegicus |