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Literature summary extracted from

  • Bourguignon, J.; Neuburger, M.; Douce, R.
    Resolution and characterization of the glycine-cleavage reaction in pea leaf mitochondria. Properties of the forward reaction catalysed by glycine decarboxylase and serine hydroxymethyltransferase (1988), Biochem. J., 255, 169-178.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.4.4.2 mitochondrion
-
 Pisum sativum 5739
-
2.1.2.1 mitochondrion
-
 Pisum sativum 5739
-
2.1.2.10 mitochondrial matrix
-
 Pisum sativum 5759
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.4.2 97000
-
 alpha2, 2 * 97000, lithium dodecyl sulfate PAGE Pisum sativum
1.4.4.2 210000
-
 gel filtration Pisum sativum
2.1.2.1 220000
-
 gel filtration Pisum sativum
2.1.2.10 45000
-
 gel filtration Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.4.2 glycine + lipoylprotein Pisum sativum lipoyl protein: H-protein, lipoamide can also act as acceptor S-aminomethyldihydrolipoylprotein + CO2
-
 ?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate Pisum sativum part of the glycine cleavage system dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.4.2 Pisum sativum
-
 pea
-
2.1.2.1 Pisum sativum
-
 pea
-
2.1.2.10 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.4.2
-
 Pisum sativum
2.1.2.1
-
 Pisum sativum
2.1.2.10 8.8fold purification Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.4.2 leaf matrix Pisum sativum
-
2.1.2.1 leaf
-
 Pisum sativum
-
2.1.2.10 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.2.1 additional information
-
-
 Pisum sativum
2.1.2.10 1.08
-
-
 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
1.4.4.2 -80°C Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.4.2 glycine + lipoylprotein lipoyl protein: H-protein, lipoamide can also act as acceptor Pisum sativum S-aminomethyldihydrolipoylprotein + CO2
-
 ?
2.1.2.1 L-serine + tetrahydrofolate
-
 Pisum sativum glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
2.1.2.10 additional information enzyme is a component of the glycine cleavage system which is composed of P-, H-, L- and T-protein, multienzyme complex Pisum sativum ?
-
 ?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate T-protein catalyzes the tetrahydrofolate-dependent step of the glycine cleavage reaction Pisum sativum dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate tetrahydrofolate-dependent enzyme Pisum sativum dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?
2.1.2.10 S-aminomethyldihydrolipoylprotein + (6S)-tetrahydrofolate part of the glycine cleavage system Pisum sativum dihydrolipoylprotein + (6R)-5,10-methylenetetrahydrofolate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.4.2 dimer alpha2, 2 * 97000, lithium dodecyl sulfate PAGE Pisum sativum
2.1.2.1 homotetramer lithium(dodecyl sulfate)-PAGE Pisum sativum
2.1.2.10 monomer 1 * 45000, lithium dodecyl sulfate PAGE Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Pisum sativum
2.1.2.10 tetrahydrofolate tetrahydrofolate-dependent enzyme Pisum sativum