BRENDA - Enzyme Database

Evidence against reduction of Cu2+ to Cu+ during dioxygen activation in a copper amine oxidase from Yeast

Mills, S.A.; Klinman, J.P.; J. Am. Chem. Soc. 122, 9897-9904 (2000)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.4.3.21
-
Ogataea angusta
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.21
0.01
-
methylamine
native enzyme
Ogataea angusta
1.4.3.21
0.68
-
methylamine
Co2+ reconstituted enzyme
Ogataea angusta
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.21
copper
copper depleted enzyme can be reconstituted with either Cu2+, Zn2+, Co2+, or Ni2+, 79% of activity is restored with Cu2+, 19% is restored with Co2+, 1.7% with Zn2+ or Ni2+
Ogataea angusta
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.21
Ogataea angusta
-
1A2V
-
1.4.3.21
Ogataea angusta 1A2V
-
1A2V
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.21
-
Ogataea angusta
Reaction
EC Number
Reaction
Commentary
Organism
1.4.3.21
RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2
role of copper in enzyme activity
Ogataea angusta
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.21
0.0193
-
copper depleted, reconstituted with Zn2+
Ogataea angusta
1.4.3.21
0.0195
-
copper depleted, reconstituted with Ni2+
Ogataea angusta
1.4.3.21
0.0571
-
copper depleted enzyme
Ogataea angusta
1.4.3.21
0.213
-
copper depleted, reconstituted with Co2+
Ogataea angusta
1.4.3.21
0.894
-
copper depleted, reconstituted with Cu2+
Ogataea angusta
1.4.3.21
1.13
-
native enzyme
Ogataea angusta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.21
methylamine + H2O + O2
-
391960
Ogataea angusta
methanal + NH3 + H2O2
-
391960
Ogataea angusta
?
1.4.3.21
methylamine + H2O + O2
-
391960
Ogataea angusta 1A2V
methanal + NH3 + H2O2
-
391960
Ogataea angusta 1A2V
?
1.4.3.21
RCH2NH2 + H2O + O2
-
391960
Ogataea angusta
RCHO + NH3 + H2O2
-
391960
Ogataea angusta
?
1.4.3.21
RCH2NH2 + H2O + O2
-
391960
Ogataea angusta 1A2V
RCHO + NH3 + H2O2
-
391960
Ogataea angusta 1A2V
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.3.21
2.08
-
methylamine
Co2+ reconstituted enzyme
Ogataea angusta
1.4.3.21
2.12
-
methylamine
native enzyme
Ogataea angusta
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.21
-
Ogataea angusta
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.21
0.01
-
methylamine
native enzyme
Ogataea angusta
1.4.3.21
0.68
-
methylamine
Co2+ reconstituted enzyme
Ogataea angusta
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.21
copper
copper depleted enzyme can be reconstituted with either Cu2+, Zn2+, Co2+, or Ni2+, 79% of activity is restored with Cu2+, 19% is restored with Co2+, 1.7% with Zn2+ or Ni2+
Ogataea angusta
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.21
-
Ogataea angusta
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.21
0.0193
-
copper depleted, reconstituted with Zn2+
Ogataea angusta
1.4.3.21
0.0195
-
copper depleted, reconstituted with Ni2+
Ogataea angusta
1.4.3.21
0.0571
-
copper depleted enzyme
Ogataea angusta
1.4.3.21
0.213
-
copper depleted, reconstituted with Co2+
Ogataea angusta
1.4.3.21
0.894
-
copper depleted, reconstituted with Cu2+
Ogataea angusta
1.4.3.21
1.13
-
native enzyme
Ogataea angusta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.21
methylamine + H2O + O2
-
391960
Ogataea angusta
methanal + NH3 + H2O2
-
391960
Ogataea angusta
?
1.4.3.21
methylamine + H2O + O2
-
391960
Ogataea angusta 1A2V
methanal + NH3 + H2O2
-
391960
Ogataea angusta 1A2V
?
1.4.3.21
RCH2NH2 + H2O + O2
-
391960
Ogataea angusta
RCHO + NH3 + H2O2
-
391960
Ogataea angusta
?
1.4.3.21
RCH2NH2 + H2O + O2
-
391960
Ogataea angusta 1A2V
RCHO + NH3 + H2O2
-
391960
Ogataea angusta 1A2V
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.4.3.21
2.08
-
methylamine
Co2+ reconstituted enzyme
Ogataea angusta
1.4.3.21
2.12
-
methylamine
native enzyme
Ogataea angusta