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Literature summary extracted from

  • Mills, S.A.; Klinman, J.P.
    Evidence against reduction of Cu2+ to Cu+ during dioxygen activation in a copper amine oxidase from Yeast (2000), J. Am. Chem. Soc., 122, 9897-9904.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.21
-
 Ogataea angusta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.21 0.01
-
 methylamine native enzyme Ogataea angusta
1.4.3.21 0.68
-
 methylamine Co2+ reconstituted enzyme Ogataea angusta

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.21 copper copper depleted enzyme can be reconstituted with either Cu2+, Zn2+, Co2+, or Ni2+, 79% of activity is restored with Cu2+, 19% is restored with Co2+, 1.7% with Zn2+ or Ni2+ Ogataea angusta

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.21 Ogataea angusta
-
 1A2V
-
1.4.3.21 Ogataea angusta 1A2V
-
 1A2V
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.21
-
 Ogataea angusta

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.3.21 RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2 role of copper in enzyme activity Ogataea angusta
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.21 0.0193
-
 copper depleted, reconstituted with Zn2+ Ogataea angusta
1.4.3.21 0.0195
-
 copper depleted, reconstituted with Ni2+ Ogataea angusta
1.4.3.21 0.0571
-
 copper depleted enzyme Ogataea angusta
1.4.3.21 0.213
-
 copper depleted, reconstituted with Co2+ Ogataea angusta
1.4.3.21 0.894
-
 copper depleted, reconstituted with Cu2+ Ogataea angusta
1.4.3.21 1.13
-
 native enzyme Ogataea angusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.21 methylamine + H2O + O2
-
 Ogataea angusta methanal + NH3 + H2O2
-
 ?
1.4.3.21 methylamine + H2O + O2
-
 Ogataea angusta 1A2V methanal + NH3 + H2O2
-
 ?
1.4.3.21 RCH2NH2 + H2O + O2
-
 Ogataea angusta RCHO + NH3 + H2O2
-
 ?
1.4.3.21 RCH2NH2 + H2O + O2
-
 Ogataea angusta 1A2V RCHO + NH3 + H2O2
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.21 2.08
-
 methylamine Co2+ reconstituted enzyme Ogataea angusta
1.4.3.21 2.12
-
 methylamine native enzyme Ogataea angusta