BRENDA - Enzyme Database

Copper amine oxidase: heterologous expression, purification, and characterization of an active enzyme in Saccharomyces cerevisiae

Cai, D.; Klinman, J.P.; Biochemistry 33, 7647-7653 (1994)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.4.3.21
expression Saccharomyces cerevisiae
Ogataea angusta
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.21
0.017
-
benzylamine
benzylamine oxidase
Ogataea angusta
1.4.3.21
0.146
-
methylamine
recombinant enzyme
Ogataea angusta
1.4.3.21
0.682
-
benzylamine
recombinant enzyme
Ogataea angusta
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.4.3.21
peroxisome
-
Ogataea angusta
5777
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.21
copper
2 mol copper/mol enzyme dimer
Ogataea angusta
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.4.3.21
Ogataea angusta
-
1A2V
-
1.4.3.21
Ogataea angusta 1A2V
-
1A2V
-
Purification (Commentary)
EC Number
Commentary
Organism
1.4.3.21
native and recombinant enzyme
Ogataea angusta
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.21
5.6
-
recombinant enzyme, methylamine oxidation
Ogataea angusta
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.21
benzylamine + H2O + O2
-
391949
Ogataea angusta
benzaldehyde + NH3 + H2O2
-
-
-
-
1.4.3.21
benzylamine + H2O + O2
-
391949
Ogataea angusta 1A2V
benzaldehyde + NH3 + H2O2
-
-
-
-
1.4.3.21
methylamine + H2O + O2
-
391949
Ogataea angusta
methanal + NH3 + H2O2
-
-
-
-
1.4.3.21
methylamine + H2O + O2
-
391949
Ogataea angusta 1A2V
methanal + NH3 + H2O2
-
-
-
-
1.4.3.21
RCH2NH2 + H2O + O2
-
391949
Ogataea angusta
RCHO + NH3 + H2O2
-
391949
Ogataea angusta
?
1.4.3.21
RCH2NH2 + H2O + O2
-
391949
Ogataea angusta 1A2V
RCHO + NH3 + H2O2
-
391949
Ogataea angusta 1A2V
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.21
2,4,5-trihydroxyphenylalanine quinone
-
Ogataea angusta
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.21
expression Saccharomyces cerevisiae
Ogataea angusta
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.4.3.21
2,4,5-trihydroxyphenylalanine quinone
-
Ogataea angusta
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.4.3.21
0.017
-
benzylamine
benzylamine oxidase
Ogataea angusta
1.4.3.21
0.146
-
methylamine
recombinant enzyme
Ogataea angusta
1.4.3.21
0.682
-
benzylamine
recombinant enzyme
Ogataea angusta
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.4.3.21
peroxisome
-
Ogataea angusta
5777
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.4.3.21
copper
2 mol copper/mol enzyme dimer
Ogataea angusta
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.4.3.21
native and recombinant enzyme
Ogataea angusta
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.4.3.21
5.6
-
recombinant enzyme, methylamine oxidation
Ogataea angusta
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.4.3.21
benzylamine + H2O + O2
-
391949
Ogataea angusta
benzaldehyde + NH3 + H2O2
-
-
-
-
1.4.3.21
benzylamine + H2O + O2
-
391949
Ogataea angusta 1A2V
benzaldehyde + NH3 + H2O2
-
-
-
-
1.4.3.21
methylamine + H2O + O2
-
391949
Ogataea angusta
methanal + NH3 + H2O2
-
-
-
-
1.4.3.21
methylamine + H2O + O2
-
391949
Ogataea angusta 1A2V
methanal + NH3 + H2O2
-
-
-
-
1.4.3.21
RCH2NH2 + H2O + O2
-
391949
Ogataea angusta
RCHO + NH3 + H2O2
-
391949
Ogataea angusta
?
1.4.3.21
RCH2NH2 + H2O + O2
-
391949
Ogataea angusta 1A2V
RCHO + NH3 + H2O2
-
391949
Ogataea angusta 1A2V
?