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Literature summary extracted from

  • Kang, J.H.; Cho, Y.D.
    Purification and properties of diamine oxidase from soybean (Glycine max) (1989), Hanguk Saenghwahakhoe Chi, 22, 361-366.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.22 0.028
-
 spermidine
-
 Glycine max
1.4.3.22 0.065
-
 spermine
-
 Glycine max
1.4.3.22 0.53
-
 putrescine
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.22 70000
-
 2 * 70000, SDS-PAGE Glycine max
1.4.3.22 145000
-
 gel filtration Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.22 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.22
-
 Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.22 axis
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.22 additional information
-
 19.6 units/mg, 1 unit is defined as the change in absorbance of 0.1 at 470 nm/min Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.22 1,4-diaminobutane + H2O + O2
-
 Glycine max 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.22 RCH2NH2 + H2O + O2
-
 Glycine max RCHO + NH3 + H2O2
-
 ?
1.4.3.22 spermidine + H2O + O2
-
 Glycine max ?
-
 ?
1.4.3.22 spermine + H2O + O2
-
 Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.22 dimer 2 * 70000, SDS-PAGE Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.22 70
-
 15 min, stable Glycine max
1.4.3.22 80
-
 15 min, complete loss of activity Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.22 7.5
-
-
 Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.3.22 5 10 pH 5 and pH 10: 50% activity Glycine max