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Literature summary extracted from

  • Takeuchi, F.; Tsubouchi, R.; Shibata, Y.
    Effect of tryptophan metabolites on the activities of rat liver pyridoxal kinase and pyridoxamine 5-phosphate oxidase in vitro (1985), Biochem. J., 227, 537-544.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.3.5 3-Hydroxyanthranilate 145% increase in pyridoxine 5-phosphate oxidase activity, 28% increase in pyridoxamine 5-phosphate oxidase activity Rattus norvegicus
1.4.3.5 3-hydroxykynurenine 125% increase in pyridoxine 5-phosphate oxidase activity, 20% increase in pyridoxamine 5-phosphate oxidase activity Rattus norvegicus
1.4.3.5 5-hydroxyindolacetate 125% increase in pyridoxine 5-phosphate oxidase activity, 9% increase in pyridoxamine 5-phosphate oxidase activity Rattus norvegicus
1.4.3.5 5-hydroxytryptamine 20% increase in pyridoxamine 5-phosphate oxidase activity Rattus norvegicus
1.4.3.5 tryptamine 27% increase in pyridoxamine 5-phosphate oxidase activity Rattus norvegicus
1.4.3.5 xanthurenate 21% increase in pyridoxine 5-phosphate oxidase activity Rattus norvegicus
2.7.1.35 NAD+ 0.5 mM, activation to 125%, activity with pyridoxine Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.5 additional information no inhibition by tryptophan metabolites Rattus norvegicus
1.4.3.5 pyridoxamine 5'-phosphate substrate inhibition above 0.0006 mM Rattus norvegicus
2.7.1.35 5-hydroxytryptamine 0.5 mM, 11% inhibition of activity with pyridoxine, 81% inhibition of activity with pyridoxal Rattus norvegicus
2.7.1.35 NADH 0.5 mM, 14% inhibition of activity with pyridoxine, 11% inhibition of activity with pyridoxal Rattus norvegicus
2.7.1.35 picolinate 0.5 mM, 95% inhibition of activity with pyridoxine, 97% inhibition of activity with pyridoxal Rattus norvegicus
2.7.1.35 pyridoxine substrate inhibition above 0.2 mM Rattus norvegicus
2.7.1.35 quinolinate 0.5 mM, 82% inhibition of activity with pyridoxine, 82% inhibition of activity with pyridoxal Rattus norvegicus
2.7.1.35 tryptamine no inhibition of activity with pyridoxine, 72% inhibition of activity with pyridoxal Rattus norvegicus
2.7.1.35 xanthurenate 0.5 mM, 19% inhibition of activity with pyridoxine, 18% inhibition of activity with pyridoxal Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.5 0.00092
-
 pyridoxine 5'-phosphate
-
 Rattus norvegicus
1.4.3.5 0.01
-
 pyridoxamine 5'-phosphate
-
 Rattus norvegicus
2.7.1.35 0.12
-
 pyridoxine 37°C Rattus norvegicus
2.7.1.35 0.19
-
 pyridoxal 37°C Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Rattus norvegicus
-
-
-
2.7.1.35 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5
-
 Rattus norvegicus
2.7.1.35
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.5 liver
-
 Rattus norvegicus
-
2.7.1.35 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.5 0.138
-
-
 Rattus norvegicus
2.7.1.35 1.27
-
 reaction with pyridoxal Rattus norvegicus
2.7.1.35 1.42
-
 reaction with pyridoxine Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Rattus norvegicus pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxine 5'-phosphate + FMN
-
 Rattus norvegicus pyridoxal 5'-phosphate + FMNH2
-
 ?
2.7.1.35 ATP + pyridoxal
-
 Rattus norvegicus ADP + pyridoxal 5'-phosphate
-
 ?
2.7.1.35 ATP + pyridoxine
-
 Rattus norvegicus ADP + pyridoxine 5'-phosphate
-
 ?