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Literature summary extracted from

  • Pistorius, E.K.; Voss, H.
    A D-amino acid oxidase from Chlorella vulgaris (1977), Biochim. Biophys. Acta, 481, 395-406.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 L-histidine D-histidine oxidation Chlorella vulgaris
1.4.3.3 p-chloromercuribenzoate
-
 Chlorella vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 Chlorella vulgaris
-
 pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-asparagine + H2O + O2 Chlorella vulgaris
-
 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamic acid + H2O + O2 Chlorella vulgaris
-
 alpha-ketoglutarate + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamine + H2O + O2 Chlorella vulgaris
-
 2-oxoglutaramate + NH3 + H2O2
-
 ?
1.4.3.3 D-histidine + H2O + O2 Chlorella vulgaris
-
 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-isoleucine + H2O + O2 Chlorella vulgaris
-
 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2 Chlorella vulgaris
-
 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2 Chlorella vulgaris
-
 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2 Chlorella vulgaris
-
 phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2 Chlorella vulgaris
-
 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2 Chlorella vulgaris
-
 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-threonine + H2O + O2 Chlorella vulgaris
-
 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-tyrosine + H2O + O2 Chlorella vulgaris
-
 4-hydroxyphenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2 Chlorella vulgaris
-
 alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
1.4.3.3 glycine + 2 H2O + O2 Chlorella vulgaris
-
 2 formic acid + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Chlorella vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 partial Chlorella vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.4.3.3 244
-
-
 Chlorella vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
 Chlorella vulgaris pyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-asparagine + H2O + O2
-
 Chlorella vulgaris 2-oxosuccinamic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamic acid + H2O + O2
-
 Chlorella vulgaris alpha-ketoglutarate + NH3 + H2O2
-
 ?
1.4.3.3 D-glutamine + H2O + O2
-
 Chlorella vulgaris 2-oxoglutaramate + NH3 + H2O2
-
 ?
1.4.3.3 D-histidine + H2O + O2
-
 Chlorella vulgaris 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.3 D-isoleucine + H2O + O2
-
 Chlorella vulgaris 3-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-leucine + H2O + O2
-
 Chlorella vulgaris 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-methionine + H2O + O2
-
 Chlorella vulgaris 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Chlorella vulgaris phenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2
-
 Chlorella vulgaris 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2
-
 Chlorella vulgaris 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-threonine + H2O + O2
-
 Chlorella vulgaris 2-oxo-3-hydroxybutyric acid + NH3 + H2O2
-
 ?
1.4.3.3 D-tyrosine + H2O + O2
-
 Chlorella vulgaris 4-hydroxyphenylpyruvic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-valine + H2O + O2
-
 Chlorella vulgaris alpha-ketoisovaleric acid + NH3 + H2O2
-
 ?
1.4.3.3 glycine + 2 H2O + O2
-
 Chlorella vulgaris 2 formic acid + NH3 + H2O2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.3 45
-
 10 min, 35% loss of activity Chlorella vulgaris
1.4.3.3 50
-
 10 min, 57% loss of activity Chlorella vulgaris
1.4.3.3 52
-
 10 min, 76% loss of activity Chlorella vulgaris
1.4.3.3 60
-
 10 min, 93% loss of activity Chlorella vulgaris
1.4.3.3 65
-
 10 min, complete loss of activity Chlorella vulgaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD flavoprotein Chlorella vulgaris