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Literature summary extracted from

  • Pollegioni, L.; Falbo, A.; Pilone, M.S.
    Specificity and kinetics of Rhodotorula gracilis D-amino acid oxidase (1992), Biochim. Biophys. Acta, 1120, 11-16.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.4.3.3 2-mercaptoethanol stabilizes Rhodotorula toruloides
1.4.3.3 changes in ionic strength, I: 16-82 mM, have only moderate effect on enzyme stability, stability maximum is 50 mM Rhodotorula toruloides
1.4.3.3 glycerol, 10%, stabilizes Rhodotorula toruloides

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 1,3-Butadien 1-carboxylic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 2-hydroxybenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 2-hydroxybutyric acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 2-ketobutyric acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 3-Bromobenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 3-chlorobenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 3-Hydroxybenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 3-methylbenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 3-Nitrobenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 4-hydroxybenzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 benzoic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 crotonic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 kojic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 nicotinic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 Picolinic acid D-phenylglycine oxidation Rhodotorula toruloides
1.4.3.3 trans-2-Pentenoic acid D-phenylglycine oxidation Rhodotorula toruloides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.18
-
O2 with D-methionine Rhodotorula toruloides
1.4.3.3 0.29
-
D-phenylalanine
-
Rhodotorula toruloides
1.4.3.3 0.3
-
D-tryptophan
-
Rhodotorula toruloides
1.4.3.3 0.39
-
D-norleucine
-
Rhodotorula toruloides
1.4.3.3 0.5
-
D-leucine
-
Rhodotorula toruloides
1.4.3.3 0.83
-
D-methionine
-
Rhodotorula toruloides
1.4.3.3 0.89
-
D-Norvaline
-
Rhodotorula toruloides
1.4.3.3 13.75
-
D-serine
-
Rhodotorula toruloides
1.4.3.3 18.89
-
D-valine
-
Rhodotorula toruloides
1.4.3.3 21.41
-
D-cysteine
-
Rhodotorula toruloides
1.4.3.3 21.47
-
D-proline
-
Rhodotorula toruloides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2 Rhodotorula toruloides
-
pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-cysteine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-thiopropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-leucine + H2O + O2 Rhodotorula toruloides
-
4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-methionine + H2O + O2 Rhodotorula toruloides
-
4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-norleucine + H2O + O2 Rhodotorula toruloides
-
2-oxohexanoate + NH3 + H2O2
-
?
1.4.3.3 D-norvaline + H2O + O2 Rhodotorula toruloides
-
2-oxopentanoate + NH3 + H2O2
-
?
1.4.3.3 D-phenylalanine + H2O + O2 Rhodotorula toruloides
-
phenylpyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-proline + H2O + O2 Rhodotorula toruloides
-
2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-serine + H2O + O2 Rhodotorula toruloides
-
2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-tryptophan + H2O + O2 Rhodotorula toruloides
-
indol-3-pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-valine + H2O + O2 Rhodotorula toruloides
-
alpha-ketoisovaleric acid + NH3 + H2O2
-
?
1.4.3.3 thiazolidine-2-carboxylic acid + H2O + O2 Rhodotorula toruloides
-
? + NH3 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rhodotorula toruloides
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.4.3.3 a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2 ping pong bi bi mechanism Rhodotorula toruloides

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
Rhodotorula toruloides pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-cysteine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-thiopropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-leucine + H2O + O2
-
Rhodotorula toruloides 4-methyl-2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-methionine + H2O + O2
-
Rhodotorula toruloides 4-methylthio-2-oxobutanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-norleucine + H2O + O2
-
Rhodotorula toruloides 2-oxohexanoate + NH3 + H2O2
-
?
1.4.3.3 D-norvaline + H2O + O2
-
Rhodotorula toruloides 2-oxopentanoate + NH3 + H2O2
-
?
1.4.3.3 D-phenylalanine + H2O + O2
-
Rhodotorula toruloides phenylpyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-proline + H2O + O2
-
Rhodotorula toruloides 2-oxopentanoic acid + NH3 + H2O2
-
?
1.4.3.3 D-serine + H2O + O2
-
Rhodotorula toruloides 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
?
1.4.3.3 D-tryptophan + H2O + O2
-
Rhodotorula toruloides indol-3-pyruvic acid + NH3 + H2O2
-
?
1.4.3.3 D-valine + H2O + O2
-
Rhodotorula toruloides alpha-ketoisovaleric acid + NH3 + H2O2
-
?
1.4.3.3 thiazolidine-2-carboxylic acid + H2O + O2
-
Rhodotorula toruloides ? + NH3 + H2O2
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.3 40 45
-
Rhodotorula toruloides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.3.3 30 50 less than 50% of maximal activity above and below Rhodotorula toruloides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 523
-
D-valine
-
Rhodotorula toruloides
1.4.3.3 721
-
D-alanine
-
Rhodotorula toruloides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.3 8 8.5
-
Rhodotorula toruloides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.3 7.3 7.9 30 min stable Rhodotorula toruloides

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD flavoprotein Rhodotorula toruloides